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Home › Dataset Library › Dissecting engineered cell types and enhancing cell fate conversion via CellNet

Dataset: Dissecting engineered cell types and enhancing cell fate conversion via CellNet

Engineering clinically relevant cells in vitro holds promise for regenerative medicine, but most protocols fail to faithfully...

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Engineering clinically relevant cells in vitro holds promise for regenerative medicine, but most protocols fail to faithfully recapitulate target cell properties. To address this, we developed CellNet, a network biology platform that determines whether engineered cells are equivalent to their target tissues, diagnoses aberrant gene regulatory networks, and prioritizes candidate transcriptional regulators to enhance engineered conversions. Using CellNet, we improved B cell to macrophage conversion, transcriptionally and functionally, by knocking down predicted B cell regulators. Analyzing conversion of fibroblasts to induced hepatocytes (iHeps), CellNet revealed an unexpected intestinal program regulated by the master regulator Cdx2. We observed functional engraftment of mouse colon by iHeps, thereby establishing their broader potential as endoderm progenitors and demonstrating direct conversion of fibroblasts into intestinal epithelium. Our studies illustrate how CellNet can be employed to improve direct conversion and to uncover unappreciated properties of engineered cells. 15 samples

Species:
mouse

Samples:
25

Source:
E-GEOD-59037

Updated:
Dec.12, 2014

Registered:
Nov.12, 2014


Factors: (via ArrayExpress)
Sample CELL TYPE DEVELOPMENTAL STAGE
GSM1424768 Induced macrophages not specified
GSM1424768 Induced macrophages not specified
GSM1424768 Induced macrophages not specified
GSM1424768 Induced macrophages not specified
GSM1424768 Induced macrophages not specified
GSM1424768 Induced macrophages not specified
GSM1424774 Independent iHep cell line not specified
GSM1424774 Independent iHep cell line not specified
GSM1424774 Independent iHep cell line not specified
GSM1424777 liver E14.5
GSM1424777 liver E14.5
GSM1424779 liver 8-week-old adult
GSM1424779 liver 8-week-old adult
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM1424783 colon 8-week-old adult
GSM1424783 colon 8-week-old adult
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM142478 iHeps not specified
GSM142478 iHeps not specified

Tags

  • cell
  • colon
  • endoderm
  • intestinal epithelium
  • macrophage

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