{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1409243": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409243": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409243": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409243": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409247": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409247": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409247": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409247": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409247": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "FACS sorted brain-derived SMC/pericyte cells"}}, {"GSM1409252": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "aorta explants"}}, {"GSM1409252": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "aorta explants"}}, {"GSM1409252": {"GENOTYPE": "NOTCH3 +/-", "ORGANISM PART": "aorta explants"}}, {"GSM1409255": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "aorta explants"}}, {"GSM1409255": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "aorta explants"}}, {"GSM1409255": {"GENOTYPE": "NOTCH3 -/-", "ORGANISM PART": "aorta explants"}}], "id": 7660, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Using a hitherto uncharacterized knockout mouse model of Notch 3, a Notch signaling receptor paralogue highly expressed in vascular SMCs,...", "geo_gse_id": "E-GEOD-58368", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 15, "tags": ["brain", "notch", "stroke"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "linking-notch-signaling-to-ischemic-stroke", "geo_id_plat": "E-GEOD-58368_A-AFFY-45", "name": "Linking Notch signaling to ischemic stroke", "created": "Nov.12, 2014", "summary": "Using a hitherto uncharacterized knockout mouse model of Notch 3, a Notch signaling receptor paralogue highly expressed in vascular SMCs, we uncover a striking susceptibility to ischemic stroke upon challenge. Cellular and molecular analyses of vascular SMCs derived from these animals associate Notch 3 activity to the expression of specific gene targets, whereas genetic rescue experiments unambiguously link Notch 3 function in vessels to the ischemic phenotype. Microarray Studies. Biotinylated cRNA samples from freshly sorted brain SMCs (four Notch 3 +/\u2212 mice and five Notch 3 \u2212/\u2212 mice) were fragmented before hybridization (15 \u03bcg each) onto mouse 430 2.0 Affymetrix chips. The chips were washed, stained by using strepavidin-phycoerytrin, and scanned the next day as described in ref. 24. For data normalization, all probe sets were scaled to a target intensity of 150. Microarray data analysis was performed by using Rosetta Resolver. All cells were from 10- to 12-week-old male mice. Gene Ontology Analyses. PANTHER software was used to define over- and underrepresented functions in the list of signature genes found by microarray analysis (25). P values were calculated by using binomial statistics.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-58368", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-58368/samples/"}