{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1405335": {"CYTOKINES": "Media Control"}}, {"GSM1405335": {"CYTOKINES": "Media Control"}}, {"GSM1405335": {"CYTOKINES": "Media Control"}}, {"GSM1405338": {"CYTOKINES": "IL-2"}}, {"GSM1405338": {"CYTOKINES": "IL-2"}}, {"GSM1405338": {"CYTOKINES": "IL-2"}}, {"GSM140534": {"CYTOKINES": "IL-4"}}, {"GSM140534": {"CYTOKINES": "IL-4"}}, {"GSM140534": {"CYTOKINES": "IL-4"}}, {"GSM1405344": {"CYTOKINES": "IL-7"}}, {"GSM1405344": {"CYTOKINES": "IL-7"}}, {"GSM1405344": {"CYTOKINES": "IL-7"}}, {"GSM1405347": {"CYTOKINES": "IL-15"}}, {"GSM1405347": {"CYTOKINES": "IL-15"}}, {"GSM1405347": {"CYTOKINES": "IL-15"}}, {"GSM1405350": {"CYTOKINES": "IL-21"}}, {"GSM1405350": {"CYTOKINES": "IL-21"}}, {"GSM1405350": {"CYTOKINES": "IL-21"}}], "id": 7655, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Analysis of how different gamma chain family cytokines influence CD8 T cell differentiation. Na\u00efve CD8 T cells were isolated from the...", "geo_gse_id": "E-GEOD-58262", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 18, "tags": ["cell", "cytokine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "impact-of-gamma-chain-cytokines-on-the-differentia", "geo_id_plat": "E-GEOD-58262_A-AFFY-45", "name": "Impact of gamma chain cytokines on the differentiation of recently antigen-activated CD8 T cells", "created": "Nov.12, 2014", "summary": "Analysis of how different gamma chain family cytokines influence CD8 T cell differentiation. Na\u00efve CD8 T cells were isolated from the spleens OT-I Thy1.1 TCR Tg mice. Whole splenocytes from wild-type C57BL/6 mice were used as stimulator cells. Purified na\u00efve wild-type OT-I (1\u00d7106/well) were stimulated with OVA peptide (SIINFEKL) pulsed (5 \u00b5g/ml) and irradiated (2,000 rads) syngeneic splenocytes (6\u00d7106/well) in 24-well plates. Forty-eight hours later, activated OT-I T cells were harvested and viable cells were enriched over a Ficoll-paque gradient and washed with cRPMI prior to being reseeded in cRPMI (5\u00d7105 cells/ml) and treated with the media supplemented with various \u03b3c cytokines (IL-2, IL-4, IL-7, IL-15 and IL-21). Experimental samples were treated with 100 ng/ml of their respective gamma chain cytokine and incubated at 37\u00baC for 24 hours.  RNA was isolated using either the RNeasy Mini kit, or a combination of TRIzol reagent and the Direct-zol RNA miniprep kit, all following manufacturer protocols. Two biological replicates for mRNA analysis were prepped using the RNeasy kit. The third replicate was prepped using the Trizol/Direct-zol approach. The quality and quantity of RNA samples was further analyzed on the Bioanalyzer. Labeled target cDNA was prepared from total RNA samples using the Ambion MessageAmp Premier protocol (3\u2019IVT assay). Each sample target was hybridized to a Mouse 430 2.0 GeneChip array. Image processing and expression analysis were performed using Affymetrix GeneChip Command Console (AGCC) v. 3.1.1 and Affymetrix Expression Console v.1.1 software, respectively. Data from all biological replicates and conditions was imported into the Affymetrix Expression Console and normalized (RMA).  RNA processing and microarray hybridization were performed by the Oregon Health & Science University Gene Microarray Shared Resource core facility in Portland, Oregon.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-58262", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-58262/samples/"}