ArrayExpress Uploader0mouseC57BL6J8 weekscontrolD12451: 45% kCAL from fatmaleC57BL6J8 weekscontrolD12451: 45% kCAL from fatmaleC57BL6J8 weekscontrolD12451: 45% kCAL from fatmaleC57BL6J8 weekslow-dose pennicillinD12451: 45% kCAL from fatmaleC57BL6J8 weekslow-dose pennicillinD12451: 45% kCAL from fatmaleC57BL6J8 weekslow-dose pennicillinD12451: 45% kCAL from fatmaleC57BL6J8 weekslow-dose pennicillinD12451: 45% kCAL from fatmaleC57BL6J30 weekscontrolnormal chow (NC, Purina Mills International Diet # 5001, 13.5% kcal from fat)maleC57BL6J30 weekscontrolnormal chow (NC, Purina Mills International Diet # 5001, 13.5% kcal from fat)maleC57BL6J30 weekscontrolnormal chow (NC, Purina Mills International Diet # 5001, 13.5% kcal from fat)maleC57BL6J30 weekslow-dose pennicillinnormal chow (NC, Purina Mills International Diet # 5001, 13.5% kcal from fat)maleC57BL6J30 weekslow-dose pennicillinnormal chow (NC, Purina Mills International Diet # 5001, 13.5% kcal from fat)maleC57BL6J30 weekslow-dose pennicillinnormal chow (NC, Purina Mills International Diet # 5001, 13.5% kcal from fat)maleC57BL6J30 weekscontrolD12451: 45% kCAL from fatmaleC57BL6J30 weekscontrolD12451: 45% kCAL from fatmaleC57BL6J30 weekscontrolD12451: 45% kCAL from fatmaleC57BL6J30 weekslow-dose pennicillinD12451: 45% kCAL from fatmaleC57BL6J30 weekslow-dose pennicillinD12451: 45% kCAL from fatmaleC57BL6J30 weekslow-dose pennicillinD12451: 45% kCAL from fatmale7650arrayexpress_sid6This SuperSeries is composed of the SubSeries listed below. Acquisition of the intestinal microbiota begins at birth, and a stable...E-GEOD-58087/profile/8773/arrayexpressuploader519bodyobesityDec.12, 2014FalseE-GEOD-58087_A-AFFY-45altering-the-intestinal-microbiota-during-a-crit-3Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequencesNov.12, 2014This SuperSeries is composed of the SubSeries listed below. Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birth, induces metabolic alterations and affects ileal expression of genes involved in immunity. LDP that is limited to early life transiently perturbs the microbiota, which is sufficient to induce sustained effects on body composition, indicating that microbiota interactions in infancy may be critical determinants of long-term host metabolic effects. In addition, LDP enhances the effect of high-fat diet induced obesity. The growth promotion phenotype is transferrable to germ-free hosts by LDP-selected microbiota, showing that the altered microbiota, not antibiotics per se, play a causal role. These studies characterize important variables in early-life microbe-host metabolic interaction and identify several taxa consistently linked with metabolic alterations. Refer to individual Serieshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-58087http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-58087/samples/