{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 4749, "factors": [{"GSM13387": {"compound": "none", "dose": "  "}}, {"GSM13387": {"compound": "none", "dose": "  "}}, {"GSM13387": {"compound": "none", "dose": "  "}}, {"GSM133874": {"compound": "low-density lipoprotein", "dose": "200"}}, {"GSM133874": {"compound": "low-density lipoprotein", "dose": "200"}}, {"GSM133874": {"compound": "low-density lipoprotein", "dose": "200"}}, {"GSM133877": {"compound": "oxidised LDL", "dose": "200"}}, {"GSM133877": {"compound": "oxidised LDL", "dose": "200"}}, {"GSM133877": {"compound": "oxidised LDL", "dose": "200"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "LDL or Ox-LDL 200ug/ml, which showed no loss of viability after a 48 hour exposure, induced a physiological and pathological...", "pubmed_id": 18182060, "geo_gse_id": "E-GEOD-5741", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["age-related macular degeneration", "lipid", "macular degeneration", "membrane", "retina"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-arpe-19-cells-treated-with-ld", "geo_id_plat": "E-GEOD-5741_A-AFFY-44", "name": "Expression data from ARPE-19 cells treated with LDL or ox-LDL", "created": "Sep.21, 2014", "summary": "LDL or Ox-LDL 200ug/ml, which showed no loss of viability after a 48 hour exposure, induced a physiological and pathological transcriptional response, respectively. LDL induced a downregulation of genes associated with cholesterol biosynthesis while ox-LDL induced transcriptional alterations in genes related to inflammation, matrix expansion, lipid metabolism and processing, and apoptosis. Pentraxin-3 was secreted into the culture medium after RPE cells were stimulated with ox-LDL, and immunohistochemically evident in Bruchs membrane of human macular samples with age-related macular degeneration. ARPE-19 cells exposed to 200ug/ml ox-LDL had a 38% apoptosis rate compared to less than 1% when exposed to LDL or untreated controls (p<0.0001). While LDL induced a physiologic response by RPE cells, a pathological phenotypic response was seen after treatment with oxidatively modified LDL. The transcriptional, biochemical, and functional data provide initial support of a role for the hypothesis that modified LDLs are one trigger for initiating events that contribute to the development of age-related macular degeneration. Keywords: treatment with non-treatment control Human ARPE-19 cells were exposed to LDL or oxidatively modified LDL (ox-LDL) for 48 hours for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine whether retina, pigment epithelial cells develop a pathologic phenotype after exposure to low density lipoproteins (LDL) that are oxidatively modified.We have made two comparsions: LDL treatment versus non-treatment; ox-LDL treatment versus non-treatment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5741", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5741/samples/"}