{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1370588": {"SORTING GATE": "CD4+ Venus-high CXCR5+ PD1-high CD44-high Dump-"}}, {"GSM1370588": {"SORTING GATE": "CD4+ Venus-high CXCR5+ PD1-high CD44-high Dump-"}}, {"GSM1370588": {"SORTING GATE": "CD4+ Venus-high CXCR5+ PD1-high CD44-high Dump-"}}, {"GSM137059": {"SORTING GATE": "CD4+ Venus-low CXCR5+ PD1-high CD44-high Dump-"}}, {"GSM137059": {"SORTING GATE": "CD4+ Venus-low CXCR5+ PD1-high CD44-high Dump-"}}, {"GSM137059": {"SORTING GATE": "CD4+ Venus-low CXCR5+ PD1-high CD44-high Dump-"}}, {"GSM1370594": {"SORTING GATE": "CD4+ CXCR5+ PD1-intermediate CD44-high Dump-"}}, {"GSM1370594": {"SORTING GATE": "CD4+ CXCR5+ PD1-intermediate CD44-high Dump-"}}, {"GSM1370596": {"SORTING GATE": "CD4+ CXCR5-low PD1-low CD44-high Dump-"}}, {"GSM1370596": {"SORTING GATE": "CD4+ CXCR5-low PD1-low CD44-high Dump-"}}, {"GSM1370598": {"SORTING GATE": "CD4+ CD44-low CD62L-high Dump-"}}, {"GSM1370598": {"SORTING GATE": "CD4+ CD44-low CD62L-high Dump-"}}], "id": 7622, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Follicular helper T (Tfh) cells access the B cell follicle to promote antibody responses, and are particularly important for germinal...", "geo_gse_id": "E-GEOD-56883", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["cell", "intermediate"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-of-tfh-cells", "geo_id_plat": "E-GEOD-56883_A-AFFY-45", "name": "Expression data of Tfh cells", "created": "Nov.12, 2014", "summary": "Follicular helper T (Tfh) cells access the B cell follicle to promote antibody responses, and are particularly important for germinal center (GC) reactions. However, the molecular mechanisms of how Tfh cells are physically associated with GCs are incompletely understood. Here we report that the sphingosine-1-phosphate receptor 2 (S1PR2) gene is highly expressed in a subpopulation of Tfh cells that localizes in GCs. S1PR2-deficient Tfh cells exhibited reduced accumulation in GCs due to their impaired retention. T cells deficient in both S1PR2 and CXCR5 were ineffective in supporting GC responses compared to T cells deficient only in CXCR5. These results suggest that S1PR2 and CXCR5 cooperatively regulate localization of Tfh cells in GCs to support GC responses. Venus-high Tfh, Venus-low Tfh, PD1-intermediate Th, PD1-low Th and na\u00efve CD4+ T cells were sorted on FACSAria from immunized S1pr2V/+ mice or control mice for RNA extraction and hybridization on Affimetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56883", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56883/samples/"}