ArrayExpress Uploader0200824hDoxycycline [0.1ug/ml], 24hBmiR2824hDoxycycline [0.1ug/ml], 24hBmiR2824hDoxycycline [0.1ug/ml], 24hBmiR2824hDoxycycline [0.1ug/ml], 24hAmiR2824hDoxycycline [0.1ug/ml], 24hAmiR2824hDoxycycline [0.1ug/ml], 24hAmiR2824hDoxycycline [0.1ug/ml], 24hBcontrol24hDoxycycline [0.1ug/ml], 24hBcontrol24hDoxycycline [0.1ug/ml], 24hBcontrol24hDoxycycline [0.1ug/ml], 24hAcontrol24hDoxycycline [0.1ug/ml], 24hAcontrol24hDoxycycline [0.1ug/ml], 24hAcontrol12hDoxycycline [0.1ug/ml], 12hBmiR2812hDoxycycline [0.1ug/ml], 12hBmiR2812hDoxycycline [0.1ug/ml], 12hBmiR2812hDoxycycline [0.1ug/ml], 12hAmiR2812hDoxycycline [0.1ug/ml], 12hAmiR2812hDoxycycline [0.1ug/ml], 12hAmiR2812hDoxycycline [0.1ug/ml], 12hBcontrol12hDoxycycline [0.1ug/ml], 12hBcontrol12hDoxycycline [0.1ug/ml], 12hBcontrol12hDoxycycline [0.1ug/ml], 12hAcontrol12hDoxycycline [0.1ug/ml], 12hAcontrol12hDoxycycline [0.1ug/ml], 12hAcontrolarrayexpress_sid4Burkitt lymphoma (BL) is a highly aggressive B cell non-Hodgkin lymphoma (B-NHL), which originates from germinal center (GC) B cells and...24843176E-GEOD-56268/profile/8773/arrayexpressuploader424burkitt lymphomacellhodgkin lymphomalinelymphomanon-hodgkin lymphomaspindleDec.12, 2014Falsemir-28-expression-in-the-burkitt-lymphoma-cell-linE-GEOD-56268_A-AFFY-44miR-28 expression in the Burkitt lymphoma cell line P3HR1Jul.10, 2014Burkitt lymphoma (BL) is a highly aggressive B cell non-Hodgkin lymphoma (B-NHL), which originates from germinal center (GC) B cells and harbors translocations deregulating the MYC oncogene. A comparative analysis of microRNAs (miRNAs) expressed in normal and malignant GC B cells identified miR-28 as significantly down-regulated in BL, as well as in other GC-derived B-NHL. We show that re-expression of miR-28 impairs cell growth and clonogenic properties of BL cells by modulating several targets including MAD2L1, a component of the spindle checkpoint whose down-regulation is essential in mediating miR-28-induced growth-arrest, and BAG1, an activator of the ERK pathway. P3HR1 Burkitt lymphoma cell line was engineered to display inducible expression of GFP alone or GFP in combination with the miR-28 precursor from the pRTS1 vector upon doxycycline treatment. Two bulk populations (A and B) were established for both the control (GFP alone) and the miR28-expressing (GFP and miR-28 precursor) cells. Cells were induced with 0.1ug/ml doxycycline for 12h or 24h. Induction was performed on each bulk population in triplicates.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56268humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56268/samples/