{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2008, "factors": [{"GSM1358030": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "B", "GENOTYPE": "miR28"}}, {"GSM1358030": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "B", "GENOTYPE": "miR28"}}, {"GSM1358030": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "B", "GENOTYPE": "miR28"}}, {"GSM1358027": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "A", "GENOTYPE": "miR28"}}, {"GSM1358027": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "A", "GENOTYPE": "miR28"}}, {"GSM1358027": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "A", "GENOTYPE": "miR28"}}, {"GSM1358024": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "B", "GENOTYPE": "control"}}, {"GSM1358024": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "B", "GENOTYPE": "control"}}, {"GSM1358024": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "B", "GENOTYPE": "control"}}, {"GSM135802": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "A", "GENOTYPE": "control"}}, {"GSM135802": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "A", "GENOTYPE": "control"}}, {"GSM135802": {"TIME": "24h", "TREATMENT": "Doxycycline [0.1ug/ml], 24h", "BULK POPULATION": "A", "GENOTYPE": "control"}}, {"GSM1358018": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "B", "GENOTYPE": "miR28"}}, {"GSM1358018": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "B", "GENOTYPE": "miR28"}}, {"GSM1358018": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "B", "GENOTYPE": "miR28"}}, {"GSM1358015": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "A", "GENOTYPE": "miR28"}}, {"GSM1358015": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "A", "GENOTYPE": "miR28"}}, {"GSM1358015": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "A", "GENOTYPE": "miR28"}}, {"GSM1358012": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "B", "GENOTYPE": "control"}}, {"GSM1358012": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "B", "GENOTYPE": "control"}}, {"GSM1358012": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "B", "GENOTYPE": "control"}}, {"GSM1358009": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "A", "GENOTYPE": "control"}}, {"GSM1358009": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "A", "GENOTYPE": "control"}}, {"GSM1358009": {"TIME": "12h", "TREATMENT": "Doxycycline [0.1ug/ml], 12h", "BULK POPULATION": "A", "GENOTYPE": "control"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Burkitt lymphoma (BL) is a highly aggressive B cell non-Hodgkin lymphoma (B-NHL), which originates from germinal center (GC) B cells and...", "pubmed_id": 24843176, "geo_gse_id": "E-GEOD-56268", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 24, "tags": ["burkitt lymphoma", "cell", "hodgkin lymphoma", "line", "lymphoma", "non-hodgkin lymphoma", "spindle"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "mir-28-expression-in-the-burkitt-lymphoma-cell-lin", "geo_id_plat": "E-GEOD-56268_A-AFFY-44", "name": "miR-28 expression in the Burkitt lymphoma cell line P3HR1", "created": "Jul.10, 2014", "summary": "Burkitt lymphoma (BL) is a highly aggressive B cell non-Hodgkin lymphoma (B-NHL), which originates from germinal center (GC) B cells and harbors translocations deregulating the MYC oncogene. A comparative analysis of microRNAs (miRNAs) expressed in normal and malignant GC B cells identified miR-28 as significantly down-regulated in BL, as well as in other GC-derived B-NHL. We show that re-expression of miR-28 impairs cell growth and clonogenic properties of BL cells by modulating several targets including MAD2L1, a component of the spindle checkpoint whose down-regulation is essential in mediating miR-28-induced growth-arrest, and BAG1, an activator of the ERK pathway. P3HR1 Burkitt lymphoma cell line was engineered to display inducible expression of GFP alone or GFP in combination with the miR-28 precursor from the pRTS1 vector upon doxycycline treatment. Two bulk populations (A and B) were established for both the control (GFP alone) and the miR28-expressing (GFP and miR-28 precursor) cells. Cells were induced with 0.1ug/ml doxycycline for 12h or 24h. Induction was performed on each bulk population in triplicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56268", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56268/samples/"}