ArrayExpress Uploader02093co-cultured with HIV infected Jurkat T cellsco-cultured with uninfected Jurkat T cellsarrayexpress_sid4The study recapitulates, through in vitro micropatterned co-cultures, interactions between HIV-infected T-lymphocytes and intestinal...19285003E-GEOD-56090/profile/8773/arrayexpressuploader12cellepithelial cellhiv infectionsurfaceDec.12, 2014FalseE-GEOD-56090_A-AFFY-44expression-data-from-ht29-intestinal-epithelial-ceExpression data from HT29 intestinal epithelial cell lineJul.11, 2014The study recapitulates, through in vitro micropatterned co-cultures, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function during HIV infection. The co-culture method simplifies observation/monitoring of the two cell types and is particularly suited for laser microdissection-based retrieval of the epithelial cells for downstream gene expressions studies. Microarrays were used to characterize changes in gene expression in HT29 epithelial cells co-cultured with Jurkat T-lymphocytes in the presence and absence of HIV infection Micropatterned co-cultures were generated of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells whereby both cell types were positioned on the same surface in a price spatial configuration (micropattern). HT29 celsl were then isolated by laser capture microdissection and used for RNA extraction and hybridization on Affymetrix U133 Plus2 microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56090humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56090/samples/