{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2093, "factors": [{"GSM1354887": {"TREATMENT": "co-cultured with HIV infected Jurkat T cells"}}, {"GSM1354886": {"TREATMENT": "co-cultured with uninfected Jurkat T cells"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The study recapitulates, through in vitro micropatterned co-cultures, interactions between HIV-infected T-lymphocytes and intestinal...", "pubmed_id": 19285003, "geo_gse_id": "E-GEOD-56090", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cell", "epithelial cell", "hiv infection", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-56090_A-AFFY-44", "slug": "expression-data-from-ht29-intestinal-epithelial-ce", "geo_gds_id": "", "name": "Expression data from HT29 intestinal epithelial cell line", "created": "Jul.11, 2014", "summary": "The study recapitulates, through in vitro micropatterned co-cultures, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function during HIV infection. The co-culture method simplifies observation/monitoring of the two cell types and is particularly suited for laser microdissection-based retrieval of the epithelial cells for downstream gene expressions studies. Microarrays were used to characterize changes in gene expression in HT29 epithelial cells co-cultured with Jurkat T-lymphocytes in the presence and absence of HIV infection Micropatterned co-cultures were generated of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells whereby both cell types were positioned on the same surface in a price spatial configuration (micropattern).  HT29 celsl were then isolated by laser capture microdissection and used for RNA extraction and hybridization on Affymetrix U133 Plus2 microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56090", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-56090/samples/"}