{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1341457": {"SAMPLE TYPE": "hybridized"}}, {"GSM1341458": {"SAMPLE TYPE": "live"}}], "id": 8755, "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "Mouse induced pluripotent stem cells (iPSCs) were derived from embryonic fibroblasts by overexpressing the Yamanaka factors Oct4, Sox2,...", "geo_gse_id": "E-GEOD-55672", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptional-analysis-of-live-and-hybridized-ip", "geo_id_plat": "E-GEOD-55672_A-AFFY-36", "name": "Transcriptional analysis of live and hybridized iPSCs", "created": "Nov.24, 2014", "summary": "Mouse induced pluripotent stem cells (iPSCs) were derived from embryonic fibroblasts by overexpressing the Yamanaka factors Oct4, Sox2, Klf4 and c-Myc, and grown in standard 2i/serum media conditions. Hybridized iPSCs were fixed with 4% paraformaldehyde; incubated for 12 hours at 30C in an RNA preserving hybridization buffer (300 mM Sodium chloride, 30mM Sodium citrate, 2.1M Ammonium sulfate, 25% formamide, 10 mM EDTA, 1 mg/ml E. Coli tRNA, 500 \u03bcg/ml BSA); and reverse cross-linked for 1 hour at 50C in with Sodium dodecyl sulfate (SDS) and Proteinase K (100 mM NaCl, 10 mM pH 8.0 Tris, 1 mM EDTA, 0.5% SDS, 500 \u03bcg/ml Proteinase K).    RNA extracted from live and hybridized iPSCs (1 sample each) was compared by microarray.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55672", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55672/samples/"}