ArrayExpress Uploader0mousehybridizedlive8755arrayexpress_sid8Mouse induced pluripotent stem cells (iPSCs) were derived from embryonic fibroblasts by overexpressing the Yamanaka factors Oct4, Sox2,...E-GEOD-55672/profile/8773/arrayexpressuploader12serumDec.12, 2014Falsetranscriptional-analysis-of-live-and-hybridized-ipE-GEOD-55672_A-AFFY-36Transcriptional analysis of live and hybridized iPSCsNov.24, 2014Mouse induced pluripotent stem cells (iPSCs) were derived from embryonic fibroblasts by overexpressing the Yamanaka factors Oct4, Sox2, Klf4 and c-Myc, and grown in standard 2i/serum media conditions. Hybridized iPSCs were fixed with 4% paraformaldehyde; incubated for 12 hours at 30C in an RNA preserving hybridization buffer (300 mM Sodium chloride, 30mM Sodium citrate, 2.1M Ammonium sulfate, 25% formamide, 10 mM EDTA, 1 mg/ml E. Coli tRNA, 500 μg/ml BSA); and reverse cross-linked for 1 hour at 50C in with Sodium dodecyl sulfate (SDS) and Proteinase K (100 mM NaCl, 10 mM pH 8.0 Tris, 1 mM EDTA, 0.5% SDS, 500 μg/ml Proteinase K). RNA extracted from live and hybridized iPSCs (1 sample each) was compared by microarray.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55672http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55672/samples/