{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1338415": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "6", "AGE": "60-69", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "3c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "12"}}, {"GSM1338414": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "1", "AGE": "60-69", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "3c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "0"}}, {"GSM1338413": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "4", "AGE": "30-39", "HISTOLOGICAL SUBTYPE": "endometrioid", "FIGO STAGE": "4", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "66"}}, {"GSM1338412": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "0", "AGE": "60-69", "HISTOLOGICAL SUBTYPE": "mucinous", "FIGO STAGE": "1c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "7"}}, {"GSM13384": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "48", "AGE": "60-69", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "3c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "547"}}, {"GSM1338410": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "6", "AGE": "50-59", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "2a", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "163"}}, {"GSM1338409": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "13", "AGE": "30-39", "HISTOLOGICAL SUBTYPE": "clear", "FIGO STAGE": "1a", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "133"}}, {"GSM1338408": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "56", "AGE": "60-69", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "3c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "31"}}, {"GSM1338407": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "0", "AGE": "50-59", "HISTOLOGICAL SUBTYPE": "clear", "FIGO STAGE": "1c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "0"}}, {"GSM1338406": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "23", "AGE": "50-59", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "3b", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "109"}}, {"GSM1338405": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "41", "AGE": "70-79", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "2c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "73"}}, {"GSM1338404": {"MICROENVIRONMENT_CD4POSITIVE_CELL_NUMBER": "1", "AGE": "70-79", "HISTOLOGICAL SUBTYPE": "serous", "FIGO STAGE": "3c", "MICROENVIRONMENT_CD8POSITIVE_CELL_NUMBER": "25"}}], "id": 2106, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "PD-L1 suppresses host immunity and promotes tumor growth. We investigated how IFN-\u03b3 regulates PD-L1 in the ovarian cancer...", "geo_gse_id": "E-GEOD-55512", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 5, "sample_count": 12, "tags": ["cancer", "cell", "epithelial cell", "genome", "line", "lymphocyte", "ovarian cancer", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-profiles-of-ovarian-cancer-regardi", "geo_id_plat": "E-GEOD-55512_A-AFFY-44", "name": "Gene-expression profiles of ovarian cancer regarding its microenvironment", "created": "Jul.11, 2014", "summary": "PD-L1 suppresses host immunity and promotes tumor growth. We investigated how IFN-\u03b3 regulates PD-L1 in the ovarian cancer microenvironment.  In clinical samples, the number of stromal CTLs in peritoneally disseminated tumors was correlated with PD-L1 expression on the tumor cells, and the lymphocyte number was significantly related to the IFN-\u03b3 signature score. In mouse models, PD-L1 was induced in peritoneal disseminated tumors, where lymphocytes were prominent, but not in subcutaneous tumors. Depleting IFNGR1 resulted in lower PD-L1 expression and longer survival in peritoneal dissemination model. Injection of IFN-\u03b3 into subcutaneous tumors increased PD-L1 expression and tumor size, and PD-L1 depletion abrogated tumor growth. These data suggest that IFN-\u03b3 works as a tumor progressor through PD-L1 induction. The source of IFN-\u03b3 in ovarian cancer microenvironment and its biological effect to the tumor cells is unclear. The immortalized human ovarian surface epithelial cell line, HOSE-E7/hTERT (HOSE) was treated with IFN-\u03b3 and expression microarray analysis was performed, and probes showing significantly higher values in IFN-\u03b3-added group were termed \u201cIFN-\u03b3 signature genes (295 probes)\u201d.  We then applied this signature to our ovarian cancer microarray data, which included 75 ovarian cancer clinical samples, by means of ss-GSEA. IFN-\u03b3 signature score was strongly correlated to the number of infiltrating CD4-positive or CD8-positive lymphocytes in the tumors. These data suggest that the IFN-\u03b3 in the ovarian cancer microenvironment is derived from lymphocytes, and an IFN-\u03b3-rich microenvironment is strongly correlated to a lymphocyte-rich microenvironment. Genome-wide transcriptional changes in human ovarian cancer tissue were observed in different tumor immunological microenvironment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55512", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55512/samples/"}