{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7563, "factors": [{"GSM1323884": {"TREATMENT": "M-CSF"}}, {"GSM1323885": {"TREATMENT": "M-CSF + dexamethasone"}}, {"GSM1323884": {"TREATMENT": "M-CSF"}}, {"GSM1323885": {"TREATMENT": "M-CSF + dexamethasone"}}, {"GSM1323884": {"TREATMENT": "M-CSF"}}, {"GSM1323885": {"TREATMENT": "M-CSF + dexamethasone"}}], "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54778", "summary_wrapped": "Glucocorticoids (GC) are used as first line therapies for generalized suppression of inflammation (e.g. allergies or autoimmune...", "pubmed_id": 24990080, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["bone", "bone marrow", "cell", "colitis", "colon", "cytokine", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "glucocorticoid-induced-gene-regulation-in-murine-b", "geo_id_plat": "E-GEOD-54778_A-AFFY-45", "name": "Glucocorticoid induced gene-regulation in murine bone marrow derived monocytes.", "created": "Nov.12, 2014", "summary": "Glucocorticoids (GC) are used as first line therapies for generalized suppression of inflammation (e.g. allergies or autoimmune diseases), but their long-term use is limited by severe side effects. Our previous work has revealed that GC induced a stable anti-inflammatory phenotype in monocytes, the glucocorticoid-stimulated monocytes (GCsM) that we now exploited for targeted GC-mediated therapeutic effects. We demonstrate that GCsM interact with T-cells in suppressing proliferation as well as cytokine release of CD8+ and especially CD4+ T-cells in vitro, and that they support generation of Foxp3+ cells.  We therefore tested their immunosuppressive potential in CD4+ T-cell-induced colitis in vivo. We found that injection of GCsM into mice with already established severe colitis abolishes the inflammation and results in significant clinical improvement within a few days. T-cells recovered from GCsM-treated mice reveal reduced secretion of pro-inflammatory cytokines IFN-\uf067 or IL-17. Furthermore, accumulation of clusters of Foxp3+ CD4+ T-cells were detectable at local sites of inflammation in their colon.   Thus, GCsM are able to modify T-cell responses in vitro and in vivo and are able to down-regulate and clinically cure an established severe T-cell mediated colitis. Bone marrow derived monocytes where cultured 16 h with media containing M-CSF (control monocytes) or media containing M-CSF and dexamethasone (GCsM). In three independent experiments, total RNA from GCsM and control monocytes was isolated and processed for microarray hybridization.", "geo_gse_id": "E-GEOD-54778", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54778/samples/"}