{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8748, "factors": [{"GSE5475GSM124314": {}}, {"GSE5475GSM124315": {}}, {"GSE5475GSM124316": {}}, {"GSE5475GSM124317": {}}, {"GSE5475GSM124318": {}}, {"GSE5475GSM124320": {}}, {"GSE5475GSM124308": {}}, {"GSE5475GSM124309": {}}, {"GSE5475GSM124310": {}}, {"GSE5475GSM124311": {}}, {"GSE5475GSM124312": {}}, {"GSE5475GSM124313": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "The peroxisome proliferator-activated receptor alpha (PPAR\u03b1) is a fatty acid-activated transcription factor that governs a variety of...", "pubmed_id": 17426115, "geo_gse_id": "E-GEOD-5475", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["cell", "fatty acid", "intestine", "jejunum", "obesity", "organ", "peroxisome", "proximal", "small intestine", "villus"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-small-intestine-6", "geo_id_plat": "E-GEOD-5475_A-AFFY-36", "name": "Transcription profiling of mouse small intestine from wild-type (129S1/SvImJ) and PPARI?-null (129S4/SvJae) anmals treated with the synthetic PPARI ligand WY14,643 (0.1% w/w) for 5 days", "created": "Nov.24, 2014", "summary": "The peroxisome proliferator-activated receptor alpha (PPAR\u03b1) is a fatty acid-activated transcription factor that governs a variety of biological processes. Little is known about the role of PPAR\u03b1 in the small intestine. Since this organ is frequently exposed to high levels of PPAR\u03b1 ligands via the diet, we set out to characterize the function of PPAR\u03b1 in small intestine using functional genomics experiments and bioinformatics tools. PPAR\u03b1 was expressed at high levels in both human and murine small intestine. Detailed analyses showed that PPAR\u03b1 was expressed highest in villus cells of proximal jejunum. Microarray analyses of total tissue samples revealed, that in addition to genes involved in fatty acid and triacylglycerol metabolism, transcription factors and enzymes connected to sterol and bile acid metabolism, including FXR and SREBP1, were specifically induced. In contrast, genes involved in cell cycle and differentiation, apoptosis, and host defense were repressed by PPAR\u03b1 activation. Additional analyses showed that intestinal PPAR\u03b1 dependent gene regulation occurred in villus cells. Functional implications of array results were corroborated by morphometric data. The repression of genes involved in proliferation and apoptosis was accompanied by a 22% increase in villus height, and a 34% increase in villus area of wild-type animals treated with WY14643. This is the first report providing a comprehensive overview of processes under control of PPAR\u03b1 in the small intestine. We show that PPAR\u03b1 is an important transcriptional regulator in small intestine, which may be of importance for the development of novel foods and therapies for obesity and inflammatory bowel diseases.  Experiment Overall Design: Pure bred wild-type (129S1/SvImJ) and PPAR\u03b1-null (129S4/SvJae) mice were treated with the synthetic PPAR\u03b1 ligand WY14,643 (0.1% w/w) for 5 days. The complete intestines were then removed and total RNA was isolated.   Experiment Overall Design: In total 4 experimental groups were present: wild type mice fed the control diet (AIN93M), wild type mice fed the control diet supplemented with 0.1% w/w WY14,643 for 5 days, PPAR\u03b1 knockout mice fed the control diet, PPAR\u03b1 knockout mice fed the control diet supplemented with 0.1% w/wWY14,643 for 5 days.  Experiment Overall Design: RNA of 3 biological replicates was hybridized to Affymetrix 430A arrays. Experiment Overall Design: Five microgram total RNA was labelled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix's protocols.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5475", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5475/samples/"}