ArrayExpress Uploader0mouseEncephalitogenic TcellsCD4+Encephalitogenic TcellsCD4+Encephalitogenic TcellsCD4+natural FoxA1TregCD4hi,PD-L1hinatural FoxA1TregCD4hi,PD-L1hinatural FoxA1TregCD4hi,PD-L1hiTregCD4+,TGFbeta+TregCD4+,TGFbeta+TregCD4+,TGFbeta+induced FoxA1TregCD4hi,PD-L1hiinduced FoxA1TregCD4hi,PD-L1hiinduced FoxA1TregCD4hi,PD-L1hi7554arrayexpress_sid6Affymetrix gene expression analysis was carried out to investigate the differences in gene profile of MBP89-101-reactive encephalitogenic...E-GEOD-54490/profile/8773/arrayexpressuploader212cellmembranesurfaceDec.12, 2014Falsefoxa1-directs-the-lineage-and-immunosuppressive-prE-GEOD-54490_A-AFFY-45FoxA1 directs the lineage and immunosuppressive properties of FoxA1+ regulatory T cells in EAE and MSNov.12, 2014Affymetrix gene expression analysis was carried out to investigate the differences in gene profile of MBP89-101-reactive encephalitogenic T cells before and after co-culture with cerebellar granular neurons (CGNs). Co-culture of MBP89-101-reactive encephalitogenic T cells with CGNs leads to generation of T cells with regulatory T cells phenotype (CD4+CD25+membrane bound TGF-b+ T cells) or a new regulatory phenotype (CD4highPD-L1high T cells). CGN-induced CD4+CD25+membrane bound TGF-b+ T regulatory cells, CD4highPD-L1high T cells were purified by FACSAria. IFN-beta induced T lymphocytes (CD4highPD-L1high T cells) were also FACSAria purified. All these populations were compared to MBP89-101-reactive encephalitogenic T cells. Samples were prepared from biological triplicates for each FACSAria sorted population. There are four different cell types, as determined by cell surface markers, in triplicate in the experiment.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54490http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54490/samples/