{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 7552, "factors": [{"GSM1314218 1": {}}, {"GSM1314219 1": {}}, {"GSM1314220 1": {}}, {"GSM1314221 1": {}}], "pop_total": 0, "platform": 6, "summary_wrapped": "Little is known about the function of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) generated from diabetics, as...", "geo_gse_id": "E-GEOD-54387", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["cell", "ischemia", "muscle", "obesity", "pluripotent stem cell", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-induced-pluripotent-stem-cell", "geo_id_plat": "E-GEOD-54387_A-AFFY-45", "name": "Expression data from induced pluripotent stem cell-derived endothelial cells from healthy and diet-induced obesity mice", "created": "Nov.12, 2014", "summary": "Little is known about the function of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) generated from diabetics, as this could potentially limit subsequent therapeutic use in this patient population. Here, we demonstrate that iPSC-ECs derived from diet-induced obesity (DIO) mice exhibit evidence of endothelial dysfunction. We also observed that mice receiving intramuscular (IM) injections of DIO iPSC-ECs had significantly decreased reperfusion following hindlimb ischemia compared to mice administered with iPSC-ECs from control mice. Hindlimb sections revealed increased muscle atrophy and presence of inflammatory cells in mice receiving iPSC-ECs from DIO mice. When pravastatin was administered to mice receiving DIO iPSC-ECs, a significant increase in reperfusion was observed, which was blunted by co-administration of L-NAME. This study is the first to provide evidence that iPSC-ECs from pre-diabetic mice exhibit signs of endothelial function, and suggest that pravastatin administration may be needed for diabetic patients receiving autologous iPSC-ECs therapy in the clinic. Four samples were analyzed, two from the healthy (control) group and two from the diet-induced obesity group", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54387", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54387/samples/"}