ArrayExpress Uploaderarrayexpress_sidmouse�129/Sv (Z.J. Chen, U.T. Southwestern)Type I Toxoplasmawild type�129/Sv (Z.J. Chen, U.T. Southwestern)Type I Toxoplasmawild type�129/Sv (Z.J. Chen, U.T. Southwestern)Type I ToxoplasmaMAVS-/-�129/Sv (Z.J. Chen, U.T. Southwestern)Type I ToxoplasmaMAVS-/-�129/Sv (Z.J. Chen, U.T. Southwestern)Type Imaf1KO Toxoplasmawild type�129/Sv (Z.J. Chen, U.T. Southwestern)Type Imaf1KO Toxoplasmawild type�129/Sv (Z.J. Chen, U.T. Southwestern)Type Imaf1KO ToxoplasmaMAVS-/-�129/Sv (Z.J. Chen, U.T. Southwestern)Type Imaf1KO ToxoplasmaMAVS-/-129/SvEv (ATCC CRL-2991)Type II Toxoplasmawild type129/SvEv (ATCC CRL-2991)Type II Toxoplasmawild type129/SvEv (ATCC CRL-2991)Type II:MAF1 Toxoplasmawild type129/SvEv (ATCC CRL-2991)Type II:MAF1 Toxoplasmawild type754806The goals of the microarray experiment were to determine the role of MAF1, the Toxoplasma gondii mediator of host mitochondrial...E-GEOD-54285/profile/8773/arrayexpressuploader312cellDec.12, 2014FalseE-GEOD-54285_A-AFFY-45analysis-of-impact-of-toxoplasma-effector-maf1-durAnalysis of impact of Toxoplasma effector MAF1 during Type II infection in WT MEFs and during Type I infection in WT and MAVS KO MEFsNov.12, 2014The goals of the microarray experiment were to determine the role of MAF1, the Toxoplasma gondii mediator of host mitochondrial association, on host cell gene expression by comparing infection of WT cells with Type II and Type II:MAF1 parasites. We also explored the role of MAF1 on host cell gene expression by comparing profiles of WT and MAVS KO MEFs infected with Type I and Type Imaf1KO parasites. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. WT MEFs were infected with Type II and Type II:MAF1 parasites at an MOI of 5. At 8 hpi RNA was harvested using TriZol. The Affymetrix IVT (16h) express kit was used for cDNA synthesis and labeling.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54285http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54285/samples/