{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1311863": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type I Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM1311863": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type I Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM1311865": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type I Toxoplasma", "GENOTYPE": "MAVS-/-"}}, {"GSM1311865": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type I Toxoplasma", "GENOTYPE": "MAVS-/-"}}, {"GSM1311867": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type Imaf1KO Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM1311867": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type Imaf1KO Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM1311869": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type Imaf1KO Toxoplasma", "GENOTYPE": "MAVS-/-"}}, {"GSM1311869": {"STRAIN BACKGROUND": "\ufffd129/Sv (Z.J. Chen, U.T. Southwestern)", "INFECTED WITH": "Type Imaf1KO Toxoplasma", "GENOTYPE": "MAVS-/-"}}, {"GSM131187": {"STRAIN BACKGROUND": "129/SvEv (ATCC CRL-2991)", "INFECTED WITH": "Type II Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM131187": {"STRAIN BACKGROUND": "129/SvEv (ATCC CRL-2991)", "INFECTED WITH": "Type II Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM1311873": {"STRAIN BACKGROUND": "129/SvEv (ATCC CRL-2991)", "INFECTED WITH": "Type II:MAF1 Toxoplasma", "GENOTYPE": "wild type"}}, {"GSM1311873": {"STRAIN BACKGROUND": "129/SvEv (ATCC CRL-2991)", "INFECTED WITH": "Type II:MAF1 Toxoplasma", "GENOTYPE": "wild type"}}], "id": 7548, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The goals of the microarray experiment were to determine the role of MAF1, the Toxoplasma gondii mediator of host mitochondrial...", "geo_gse_id": "E-GEOD-54285", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 12, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "analysis-of-impact-of-toxoplasma-effector-maf1-dur", "geo_id_plat": "E-GEOD-54285_A-AFFY-45", "name": "Analysis of impact of Toxoplasma effector MAF1 during Type II infection in WT MEFs and during Type I  infection in WT and MAVS KO MEFs", "created": "Nov.12, 2014", "summary": "The goals of the microarray experiment were to determine the role of MAF1, the Toxoplasma gondii mediator of host mitochondrial association, on host cell gene expression by comparing infection of WT cells with Type II and Type II:MAF1 parasites. We also explored the role of MAF1 on host cell gene expression by comparing profiles of WT and MAVS KO MEFs infected with Type I and Type Imaf1KO parasites. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. WT MEFs were infected with Type II and Type II:MAF1 parasites at an MOI of 5. At 8 hpi RNA was harvested using TriZol. The Affymetrix IVT (16h) express kit was used for cDNA synthesis and labeling.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54285", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-54285/samples/"}