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Home › Dataset Library › Expression data from mouse bone marrow cells expressing renin driven expression of green fluorescent protein.

Dataset: Expression data from mouse bone marrow cells expressing renin driven expression of green fluorescent protein.

Local renin antiotensin systems have been identified for many extra-renal sites. Bone marrow has been proposed as one such site, although...

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Local renin antiotensin systems have been identified for many extra-renal sites. Bone marrow has been proposed as one such site, although the nature of the renin-expressing cell type(s) has not been established. Affymetrix microarrays were used to characterize the expression profile of renin-expressing GFP positive cells. Green fluorescent protein positive cells were sorted from whole bone marrow collected from adult transgenic mice. Bone marrow from several mice was collected and pooled on the day of a FACS sort. A portion of this was reserved for RNA extraction while the remainder was sorted. RNA was prepared with Trizol. Bone marrow collection and sorting was performed 3 times so that microarrays could be run in triplicate.

Species:
mouse

Samples:
6

Source:
E-GEOD-53916

Updated:
Dec.12, 2014

Registered:
Nov.12, 2014


Factors: (via ArrayExpress)
Sample CELL TYPE
GSM1303498 control
GSM1303499 renin-expression
GSM1303498 control
GSM1303499 renin-expression
GSM1303498 control
GSM1303499 renin-expression

Tags

  • bone
  • bone marrow
  • cell
  • protein

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