ArrayExpress Uploader0humanCells were collected after 96hr of cultureuntreatedcontrolCells were collected after 96hr of cultureuntreatedcontrolCells were collected after 96hr of cultureuntreatedcontrolCells were collected 96hr after siRNA transfectionsiRNA containing a scramble sequence96hr transfectionCells were collected 96hr after siRNA transfectionsiRNA containing a scramble sequence96hr transfectionCells were collected 96hr after siRNA transfectionsiRNA containing a scramble sequence96hr transfectionCells were collected 48hr after siRNA transfectionsiRNA containing a scramble sequence48hr transfectionCells were collected 48hr after siRNA transfectionsiRNA containing a scramble sequence48hr transfectionCells were collected 48hr after siRNA transfectionsiRNA containing a scramble sequence48hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting BRMS196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting BRMS148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting BRMS196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting BRMS148hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting KISS148hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting KISS148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting RRM196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting RRM148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting RRM196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting RRM148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting PEBP196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting PEBP148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting PEBP196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting PEBP148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CD4496hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CD4448hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting DRG196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting DRG148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting DRG196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting DRG148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting AKAP1296hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting AKAP1296hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting ARGHDIB96hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting ARGHDIB48hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting ARGHDIB96hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting ARGHDIB48hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAPK1496hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAPK1448hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAPK1496hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAPK1448hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAP2K796hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAP2K748hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAP2K796hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAP2K748hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAP2K696hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAP2K648hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAP2K696hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAP2K648hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting GSN96hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting GSN48hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting GSN96hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting GSN48hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAP2K496hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAP2K448hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting MAP2K496hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting MAP2K448hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CDH1196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CDH1148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CDH1196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CDH1148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CDH296hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CDH296hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CDH248hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CDH148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CDH196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CDH148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CASP896hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CASP848hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CASP896hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CASP848hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting NME196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting NME148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting NME196hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting NME148hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CD8296hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CD8248hr transfectionCells were collected 96hr after siRNA transfectionsiRNA targeting CD8296hr transfectionCells were collected 48hr after siRNA transfectionsiRNA targeting CD8248hr transfection2180arrayexpress_sid4Metastasis suppressor genes (MSGs) have contributed to an understanding of regulatory pathways unique to the lethal metastatic process....E-GEOD-53668/profile/8773/arrayexpressuploader377breastbreast cancerbreast carcinomacancercarcinomacelllineDec.12, 2014Falseidentification-and-validation-of-genes-with-expresE-GEOD-53668_A-AFFY-44Identification and Validation of Genes with Expression Patterns Inverse to Multiple Metastasis Suppressor Genes in Breast Cancer Cell LinesJul.11, 2014Metastasis suppressor genes (MSGs) have contributed to an understanding of regulatory pathways unique to the lethal metastatic process. When re-expressed in experimental models, MSGs block cancer spread to, and colonization of distant sites without affecting primary tumor formation. On a single MSG basis, genes have been identified with expression patterns inverse to a MSG, and found to encode functional, druggable signaling pathways. We now hypothesize that common signaling pathways mediate the effects of many MSGs. By gene expression profiling of human MCF7 breast carcinoma cells expressing a scrambled siRNA or siRNAs to each of 19 validated MSGs (NME1, BRMS1, CD82, CDH1, CDH2, CDH11, CASP8, MAP2K4, MAP2K6, MAP2K7, MAPK14, GSN, ARHGDIB, AKAP12, DRG1, CD44, PEBP1, RRM1, KISS1), we identified genes whose expression was significantly opposite to at least five MSGs. We used microarrays to evaluate the modification in gene expression profile after donregulation of multiple metastasis suppressors (NME1, BRMS1, CD82, CDH1, CDH2, CDH11, CASP8, MAP2K4, MAP2K6, MAP2K7, MAPK14, GSN, ARHGDIB, AKAP12, DRG1, CD44, PEBP1, RRM1, KISS1) in the breast cancer cell line, MCF7. MCF7 cell line was transfected with siRNA targeting each of 19 metastasis suppressors (NME1, BRMS1, CD82, CDH1, CDH2, CDH11, CASP8, MAP2K4, MAP2K6, MAP2K7, MAPK14, GSN, ARHGDIB, AKAP12, DRG1, CD44, PEBP1, RRM1, KISS1). Two siRNA sequences (siRNA1 and siRNA2) were used for each metastasis suppressor. Two time points (48hr and 96hr) were considered as end of transfection in order to measure early and late effects of the metastasis suppressors downregulation.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53668http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53668/samples/