Dataset: Identification and Validation of Genes with Expression Patterns Inverse to Multiple Metastasis Suppressor Genes in Breast Cancer Cell Lines

Metastasis suppressor genes (MSGs) have contributed to an understanding of regulatory pathways unique to the lethal metastatic process....

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Metastasis suppressor genes (MSGs) have contributed to an understanding of regulatory pathways unique to the lethal metastatic process. When re-expressed in experimental models, MSGs block cancer spread to, and colonization of distant sites without affecting primary tumor formation. On a single MSG basis, genes have been identified with expression patterns inverse to a MSG, and found to encode functional, druggable signaling pathways. We now hypothesize that common signaling pathways mediate the effects of many MSGs. By gene expression profiling of human MCF7 breast carcinoma cells expressing a scrambled siRNA or siRNAs to each of 19 validated MSGs (NME1, BRMS1, CD82, CDH1, CDH2, CDH11, CASP8, MAP2K4, MAP2K6, MAP2K7, MAPK14, GSN, ARHGDIB, AKAP12, DRG1, CD44, PEBP1, RRM1, KISS1), we identified genes whose expression was significantly opposite to at least five MSGs. We used microarrays to evaluate the modification in gene expression profile after donregulation of multiple metastasis suppressors (NME1, BRMS1, CD82, CDH1, CDH2, CDH11, CASP8, MAP2K4, MAP2K6, MAP2K7, MAPK14, GSN, ARHGDIB, AKAP12, DRG1, CD44, PEBP1, RRM1, KISS1) in the breast cancer cell line, MCF7. MCF7 cell line was transfected with siRNA targeting each of 19 metastasis suppressors (NME1, BRMS1, CD82, CDH1, CDH2, CDH11, CASP8, MAP2K4, MAP2K6, MAP2K7, MAPK14, GSN, ARHGDIB, AKAP12, DRG1, CD44, PEBP1, RRM1, KISS1). Two siRNA sequences (siRNA1 and siRNA2) were used for each metastasis suppressor. Two time points (48hr and 96hr) were considered as end of transfection in order to measure early and late effects of the metastasis suppressors downregulation.

Species:: human
Samples:: 77
Source:: E-GEOD-53668
Updated:: Dec.12, 2014
Registered:: Jul.11, 2014

Factors: (via ArrayExpress)

Sample	SIRNA TRANSFECTION TIME	TREATMENT	TIME
GSM1298687	Cells were collected after 96hr of culture	untreated	control
GSM1298687	Cells were collected after 96hr of culture	untreated	control
GSM1298687	Cells were collected after 96hr of culture	untreated	control
GSM1298684	Cells were collected 96hr after siRNA transfection	siRNA containing a scramble sequence	96hr transfection
GSM1298684	Cells were collected 96hr after siRNA transfection	siRNA containing a scramble sequence	96hr transfection
GSM1298684	Cells were collected 96hr after siRNA transfection	siRNA containing a scramble sequence	96hr transfection
GSM129868	Cells were collected 48hr after siRNA transfection	siRNA containing a scramble sequence	48hr transfection
GSM129868	Cells were collected 48hr after siRNA transfection	siRNA containing a scramble sequence	48hr transfection
GSM129868	Cells were collected 48hr after siRNA transfection	siRNA containing a scramble sequence	48hr transfection
GSM1298678	Cells were collected 96hr after siRNA transfection	siRNA targeting BRMS1	96hr transfection
GSM1298677	Cells were collected 48hr after siRNA transfection	siRNA targeting BRMS1	48hr transfection
GSM1298678	Cells were collected 96hr after siRNA transfection	siRNA targeting BRMS1	96hr transfection
GSM1298677	Cells were collected 48hr after siRNA transfection	siRNA targeting BRMS1	48hr transfection
GSM1298674	Cells were collected 48hr after siRNA transfection	siRNA targeting KISS1	48hr transfection
GSM1298674	Cells were collected 48hr after siRNA transfection	siRNA targeting KISS1	48hr transfection
GSM1298672	Cells were collected 96hr after siRNA transfection	siRNA targeting RRM1	96hr transfection
GSM129867	Cells were collected 48hr after siRNA transfection	siRNA targeting RRM1	48hr transfection
GSM1298672	Cells were collected 96hr after siRNA transfection	siRNA targeting RRM1	96hr transfection
GSM129867	Cells were collected 48hr after siRNA transfection	siRNA targeting RRM1	48hr transfection
GSM1298668	Cells were collected 96hr after siRNA transfection	siRNA targeting PEBP1	96hr transfection
GSM1298667	Cells were collected 48hr after siRNA transfection	siRNA targeting PEBP1	48hr transfection
GSM1298668	Cells were collected 96hr after siRNA transfection	siRNA targeting PEBP1	96hr transfection
GSM1298667	Cells were collected 48hr after siRNA transfection	siRNA targeting PEBP1	48hr transfection
GSM1298664	Cells were collected 96hr after siRNA transfection	siRNA targeting CD44	96hr transfection
GSM1298663	Cells were collected 48hr after siRNA transfection	siRNA targeting CD44	48hr transfection
GSM1298662	Cells were collected 96hr after siRNA transfection	siRNA targeting DRG1	96hr transfection
GSM129866	Cells were collected 48hr after siRNA transfection	siRNA targeting DRG1	48hr transfection
GSM1298662	Cells were collected 96hr after siRNA transfection	siRNA targeting DRG1	96hr transfection
GSM129866	Cells were collected 48hr after siRNA transfection	siRNA targeting DRG1	48hr transfection
GSM1298658	Cells were collected 96hr after siRNA transfection	siRNA targeting AKAP12	96hr transfection
GSM1298658	Cells were collected 96hr after siRNA transfection	siRNA targeting AKAP12	96hr transfection
GSM1298656	Cells were collected 96hr after siRNA transfection	siRNA targeting ARGHDIB	96hr transfection
GSM1298655	Cells were collected 48hr after siRNA transfection	siRNA targeting ARGHDIB	48hr transfection
GSM1298656	Cells were collected 96hr after siRNA transfection	siRNA targeting ARGHDIB	96hr transfection
GSM1298655	Cells were collected 48hr after siRNA transfection	siRNA targeting ARGHDIB	48hr transfection
GSM1298652	Cells were collected 96hr after siRNA transfection	siRNA targeting MAPK14	96hr transfection
GSM129865	Cells were collected 48hr after siRNA transfection	siRNA targeting MAPK14	48hr transfection
GSM1298652	Cells were collected 96hr after siRNA transfection	siRNA targeting MAPK14	96hr transfection
GSM129865	Cells were collected 48hr after siRNA transfection	siRNA targeting MAPK14	48hr transfection
GSM1298648	Cells were collected 96hr after siRNA transfection	siRNA targeting MAP2K7	96hr transfection
GSM1298647	Cells were collected 48hr after siRNA transfection	siRNA targeting MAP2K7	48hr transfection
GSM1298648	Cells were collected 96hr after siRNA transfection	siRNA targeting MAP2K7	96hr transfection
GSM1298647	Cells were collected 48hr after siRNA transfection	siRNA targeting MAP2K7	48hr transfection
GSM1298644	Cells were collected 96hr after siRNA transfection	siRNA targeting MAP2K6	96hr transfection
GSM1298643	Cells were collected 48hr after siRNA transfection	siRNA targeting MAP2K6	48hr transfection
GSM1298644	Cells were collected 96hr after siRNA transfection	siRNA targeting MAP2K6	96hr transfection
GSM1298643	Cells were collected 48hr after siRNA transfection	siRNA targeting MAP2K6	48hr transfection
GSM1298640	Cells were collected 96hr after siRNA transfection	siRNA targeting GSN	96hr transfection
GSM1298639	Cells were collected 48hr after siRNA transfection	siRNA targeting GSN	48hr transfection
GSM1298640	Cells were collected 96hr after siRNA transfection	siRNA targeting GSN	96hr transfection
GSM1298639	Cells were collected 48hr after siRNA transfection	siRNA targeting GSN	48hr transfection
GSM1298636	Cells were collected 96hr after siRNA transfection	siRNA targeting MAP2K4	96hr transfection
GSM1298635	Cells were collected 48hr after siRNA transfection	siRNA targeting MAP2K4	48hr transfection
GSM1298636	Cells were collected 96hr after siRNA transfection	siRNA targeting MAP2K4	96hr transfection
GSM1298635	Cells were collected 48hr after siRNA transfection	siRNA targeting MAP2K4	48hr transfection
GSM1298632	Cells were collected 96hr after siRNA transfection	siRNA targeting CDH11	96hr transfection
GSM129863	Cells were collected 48hr after siRNA transfection	siRNA targeting CDH11	48hr transfection
GSM1298632	Cells were collected 96hr after siRNA transfection	siRNA targeting CDH11	96hr transfection
GSM129863	Cells were collected 48hr after siRNA transfection	siRNA targeting CDH11	48hr transfection
GSM1298628	Cells were collected 96hr after siRNA transfection	siRNA targeting CDH2	96hr transfection
GSM1298628	Cells were collected 96hr after siRNA transfection	siRNA targeting CDH2	96hr transfection
GSM1298626	Cells were collected 48hr after siRNA transfection	siRNA targeting CDH2	48hr transfection
GSM1298625	Cells were collected 48hr after siRNA transfection	siRNA targeting CDH1	48hr transfection
GSM1298624	Cells were collected 96hr after siRNA transfection	siRNA targeting CDH1	96hr transfection
GSM1298625	Cells were collected 48hr after siRNA transfection	siRNA targeting CDH1	48hr transfection
GSM1298622	Cells were collected 96hr after siRNA transfection	siRNA targeting CASP8	96hr transfection
GSM129862	Cells were collected 48hr after siRNA transfection	siRNA targeting CASP8	48hr transfection
GSM1298622	Cells were collected 96hr after siRNA transfection	siRNA targeting CASP8	96hr transfection
GSM129862	Cells were collected 48hr after siRNA transfection	siRNA targeting CASP8	48hr transfection
GSM1298618	Cells were collected 96hr after siRNA transfection	siRNA targeting NME1	96hr transfection
GSM1298617	Cells were collected 48hr after siRNA transfection	siRNA targeting NME1	48hr transfection
GSM1298618	Cells were collected 96hr after siRNA transfection	siRNA targeting NME1	96hr transfection
GSM1298617	Cells were collected 48hr after siRNA transfection	siRNA targeting NME1	48hr transfection
GSM1298614	Cells were collected 96hr after siRNA transfection	siRNA targeting CD82	96hr transfection
GSM1298613	Cells were collected 48hr after siRNA transfection	siRNA targeting CD82	48hr transfection
GSM1298614	Cells were collected 96hr after siRNA transfection	siRNA targeting CD82	96hr transfection
GSM1298613	Cells were collected 48hr after siRNA transfection	siRNA targeting CD82	48hr transfection