{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1295146": {"CELL TYPE": "AFECs"}}, {"GSM1295147": {"CELL TYPE": "VAFECs"}}, {"GSM1295148": {"CELL TYPE": "EPCAM+CPM+ VAFECs"}}, {"GSM1295149": {"CELL TYPE": "EPCAM+CPM- VAFECs"}}, {"GSM1295148": {"CELL TYPE": "EPCAM+CPM+ VAFECs"}}, {"GSM1295149": {"CELL TYPE": "EPCAM+CPM- VAFECs"}}, {"GSM1295148": {"CELL TYPE": "EPCAM+CPM+ VAFECs"}}, {"GSM1295149": {"CELL TYPE": "EPCAM+CPM- VAFECs"}}, {"GSM1295154": {"CELL TYPE": "CPM+ VAFECs"}}], "id": 4721, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Specific surface marker for NKX2-1+ VAFECs may be helpful for isolating a homogeneous population of alveolar epithelial progenitor cells...", "geo_gse_id": "E-GEOD-53513", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["lung", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-of-hipsc-derived-anterior-foregut", "geo_id_plat": "E-GEOD-53513_A-AFFY-44", "name": "Expression data of  hiPSC-derived anterior foregut endoderm cells (AFECs), ventral anterior foregut cells (VAFECs), EpCAM+CPM+ and EpCAM+CPM- cells and CPM+ cells sorted from VAFECs", "created": "Sep.21, 2014", "summary": "Specific surface marker for NKX2-1+ VAFECs may be helpful for isolating a homogeneous population of alveolar epithelial progenitor cells and distinguishing the differentiation from a thyroid lineage to a lung lineage. In order to identify specific markers of VAFECs, a microarray analysis was performed to compare the global gene expression patterns between AFECs and VAFECs in 201B7 hiPSCs. We hypothesized that NKX2-1+ cells could be purified by sorting CPM+ VAFECs. After dissociating VAFECs cells on day 14 with Accutase, FACS was performed using anti-EPCAM and anti-CPM antibodies. EPCAM+CPM+ and EPCAM+CPM- cells were then sorted, and the global gene expression patterns of these two populations were examined using a microarray analysis. In addition, MACS was performed to obtain CPM+ cells for comparison. We extracted total RNA from hiPSCs-derived AFECs, VAFECs, EPCAM+CPM+ and EPCAM+CPM- VAFECs and CPM+ VAFECs and hybridized them to Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53513", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53513/samples/"}