ArrayExpress Uploaderarrayexpress_sid7526lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-+lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-+lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-+lean (C57BL/6J Lep +/+)Macrophage of F4/80+, CD11b+, CD11c-+obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-+obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-+obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-+obese leptin-deficient (C57BL/6J Lep ob/ob)Macrophage of F4/80+, CD11b+, CD11c-+06In mammals, expansion of adipose tissue mass induces accumulation of adipose tissue macrophages (ATMs). We isolated CD11c- (FB) and...24315368E-GEOD-53403/profile/8773/arrayexpressuploader216adipose tissuegenomeDec.12, 2014FalseE-GEOD-53403_A-AFFY-45expression-data-from-mouse-adipose-tissue-macrophaExpression data from mouse adipose tissue macrophageNov.12, 2014In mammals, expansion of adipose tissue mass induces accumulation of adipose tissue macrophages (ATMs). We isolated CD11c- (FB) and CD11c+ (FBC) perigonadal ATMs from SVCs of lean (C57BL/6J Lep +/+) and obese leptin-deficient (C57BL/6J Lep ob/ob) mice. We used expression microarrays to generate transcription profiles of perigonadal ATMs from lean (C57BL/6J Lep +/+) and obese (C57BL/6J Lep ob/ob) mice. Profiling purified FBs and FBCs, we identified 521 transcripts whose expression was differentially (nominal p-value < 0.01) expressed between FBs from lean and obese mice and 1509 genes whose expression was differentially (nominal p-value <0.01) expressed between FBC from lean and obese mice RNA was isolated from sorted FBC (F4/80+, CD11b+, CD11c+) cells and FB ( F4/80+, CD11b+, CD11c-) cells and using RNeasy micro-kits (Qiagen), using a PicoPure RNA isolation kit then amplified two-rounds. Labeled cRNA Mouse Genome 430 2.0 arrays (purified FB and FBC adipose tissue macrophages. There was a total of sixteen samples. FB and FBC populations were isolated from 4 lean and 4 obese mice.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53403mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53403/samples/