{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 7526, "factors": [{"GSM1290903": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM1290903": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM1290903": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM1290903": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM1290907": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM1290907": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM1290907": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM1290907": {"GENOTYPE": "lean (C57BL/6J Lep +/+)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM12909": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM12909": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM12909": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM12909": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-"}}, {"GSM1290915": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM1290915": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM1290915": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}, {"GSM1290915": {"GENOTYPE": "obese leptin-deficient (C57BL/6J Lep ob/ob)", "CELL TYPE": "Macrophage of F4/80+, CD11b+, CD11c-+"}}], "pop_total": 0, "platform": 6, "summary_wrapped": "In mammals, expansion of adipose tissue mass induces accumulation of adipose tissue macrophages (ATMs). We isolated CD11c- (FB) and...", "pubmed_id": 24315368, "geo_gse_id": "E-GEOD-53403", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 16, "tags": ["adipose tissue", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-53403_A-AFFY-45", "slug": "expression-data-from-mouse-adipose-tissue-macropha", "geo_gds_id": "", "name": "Expression data from mouse adipose tissue macrophage", "created": "Nov.12, 2014", "summary": "In mammals, expansion of adipose tissue mass induces accumulation of adipose tissue macrophages (ATMs). We isolated CD11c- (FB) and CD11c+ (FBC) perigonadal ATMs from SVCs of lean (C57BL/6J Lep +/+) and obese leptin-deficient (C57BL/6J Lep ob/ob) mice. We used expression microarrays to generate transcription profiles of perigonadal ATMs from lean (C57BL/6J Lep +/+) and obese (C57BL/6J Lep ob/ob) mice. Profiling purified FBs and FBCs, we identified 521 transcripts whose expression was differentially (nominal p-value < 0.01) expressed between FBs from lean and obese mice and 1509 genes whose expression was differentially (nominal p-value <0.01) expressed between FBC from lean and obese mice RNA was isolated from sorted FBC (F4/80+, CD11b+, CD11c+) cells and FB ( F4/80+, CD11b+, CD11c-) cells and using RNeasy micro-kits (Qiagen), using a PicoPure RNA isolation kit then amplified two-rounds. Labeled cRNA Mouse Genome 430 2.0 arrays (purified FB and FBC adipose tissue macrophages.  There was a total of sixteen samples. FB and FBC populations were isolated from 4 lean and 4 obese mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53403", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53403/samples/"}