{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM128797": {"GENOTYPE": "miR92 overexpression"}}, {"GSM128797": {"GENOTYPE": "miR92 overexpression"}}, {"GSM128797": {"GENOTYPE": "miR92 overexpression"}}, {"GSM1287974": {"GENOTYPE": "control"}}, {"GSM1287974": {"GENOTYPE": "control"}}, {"GSM1287974": {"GENOTYPE": "control"}}], "id": 7516, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "miR-92 enhances c-Myc induced apoptosis. In the R26MER/MER mouse embryonic fibroblasts (MEFs), a switchable variant of Myc, MycERT2, was...", "pubmed_id": 24137534, "geo_gse_id": "E-GEOD-53225", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-mir-92-over-expressing-r26mer", "geo_id_plat": "E-GEOD-53225_A-AFFY-45", "name": "Expression data from miR-92 over-expressing R26MER/MER mouse embryonic fibroblast (MEFs)", "created": "Nov.12, 2014", "summary": "miR-92 enhances c-Myc induced apoptosis. In the R26MER/MER mouse embryonic fibroblasts (MEFs), a switchable variant of Myc, MycERT2, was knocked into the genomic region downstream of the constitutive Rosa26 promoter, allowing acute activation of c-Myc by 4-OHT-induced nuclear translocation. This in vitro system nicely recapitulates c-Myc-induced apoptosis, as activated MycERT2 induces strong p53-dependent apoptosis in response to serum starvation. Enforced miR-92 expression in three independent R26MER/MER MEF lines significantly enhanced Myc-induced apoptosis. We used microarrays to investigate the molecular mechanism underlying miR-92 functions. Three independent R26MER/MER MEF lines were infected by MSCV vector alone or by MSCV vector encoding miR-92. These MEFs were serum starved and 4-OHT treated to trigger strong Myc-induced apoptosis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53225", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-53225/samples/"}