ArrayExpress Uploader0mouseethanol10^-9 M aldosterone10^-7 M aldosterone10^-7 M aldosterone and pretreatment of 5 x 10^-6 M spironolactone7502arrayexpress_sid6Target gene of mineralocorticoid receptor (MR) is comparatively unknown, although distal convoluted tubule (DCT) expresses MR in in vivo....E-GEOD-52686/profile/8773/arrayexpressuploader14celldistaldistal convoluted tubulelineDec.12, 2014Falseexpression-data-from-mdct-cell-line-over-expressinE-GEOD-52686_A-AFFY-45Expression data from mDCT cell-line over-expressing hMRNov.12, 2014Target gene of mineralocorticoid receptor (MR) is comparatively unknown, although distal convoluted tubule (DCT) expresses MR in in vivo. We used microarray and immortalized murine DCT cell-line overexpressing human MR with treatment of aldosterone to elucidate target genes of MR in DCT. mDCT overexpresses human MR by lipofection and is treated for 3 hours with ethanol (g1), or 10^-9 M aldosterone (g2), or 10^-7 M aldosterone (g3), or 10^-7 M aldosterone with pretreatment of 5 x 10^-6 M spironolactone (g4) for 2 hours.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52686http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52686/samples/