{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE5245GSM118686": {}}, {"GSE5245GSM118687": {}}, {"GSE5245GSM118689": {}}, {"GSE5245GSM118681": {}}, {"GSE5245GSM118682": {}}, {"GSE5245GSM118684": {}}, {"GSE5245GSM118668": {}}, {"GSE5245GSM118669": {}}, {"GSE5245GSM118665": {}}, {"GSE5245GSM118666": {}}, {"GSE5245GSM118667": {}}, {"GSE5245GSM118677": {}}, {"GSE5245GSM118679": {}}, {"GSE5245GSM118671": {}}, {"GSE5245GSM118673": {}}, {"GSE5245GSM118674": {}}], "id": 7496, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "These experiments were done to compare the gene expression profiles in CD4+ T cells responding to antigen presented by dendritic cells...", "geo_gse_id": "E-GEOD-5245", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 16, "tags": ["dendritic", "lymph", "lymph node"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-5245_A-AFFY-45", "slug": "transcription-profiling-of-mouse-cd4-t-cells-respo", "geo_gds_id": "", "name": "Transcription profiling of mouse CD4+ T cells responding to transient or persistent antigen presented by dendritic cells in vivo", "created": "Nov.12, 2014", "summary": "These experiments were done to compare the gene expression profiles in CD4+ T cells responding to antigen presented by dendritic cells transiently or persistently. Some treatments include the activation of the dendritic cells by CD40 engagement.  Experiment Overall Design: 2-4x106 lymph node cells from AND TCR-transgenic animals that expressed the congenic marker CD90.1 were transferred into double-transgenic (Ii-rTA/TIM) recipient animals that expressed the corresponding peptide from moth cytochrome c under a doxycycline-controllable promoter and were FACS-sorted 60 hours later. Recipients expressed the antigen not at all (\u201cctrl\u201d), transiently (\u201cshort\u201d) or throughout the experiment (\u201clong\u201d) due to different doxycycline treatment protocols. A second variable tested was the activation status of the dendritic cells: recipients were either injected by the stimulatory antibody FGK45.5 targeting CD40 (\u201cFGK\u201d) or PBS (\u201cPBS\u201d).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5245", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5245/samples/"}