{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1258465": {"AGE": "7-11sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41- 23GFP- cells from E8.5 conceptus"}}, {"GSM1258466": {"AGE": "4-9sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41- 23GFP- cells from E8.5 conceptus"}}, {"GSM1258466": {"AGE": "4-9sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41- 23GFP- cells from E8.5 conceptus"}}, {"GSM1258468": {"AGE": "7-11sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41- 23GFP+ cells from E8.5 conceptus"}}, {"GSM1258469": {"AGE": "4-9sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41- 23GFP+ cells from E8.5 conceptus"}}, {"GSM1258469": {"AGE": "4-9sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41- 23GFP+ cells from E8.5 conceptus"}}, {"GSM125847": {"AGE": "7-11sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41+ 23GFP+ cells from E8.5 conceptus"}}, {"GSM1258472": {"AGE": "4-9sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41+ 23GFP+ cells from E8.5 conceptus"}}, {"GSM1258472": {"AGE": "4-9sp", "CELL TYPE": "VE-Cad+ Ter119- CD45- CD41+ 23GFP+ cells from E8.5 conceptus"}}], "id": 7483, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "During development a specialised subset of endothelial cells, the haemogenic endothelium, undergo an endothelial-to-haematopoietic...", "geo_gse_id": "E-GEOD-52075", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 9, "tags": ["cell", "cone", "endothelium", "line", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-52075_A-AFFY-45", "slug": "expression-data-of-the-endothelial-to-hematopoieti", "geo_gds_id": "", "name": "Expression data of the endothelial-to-hematopoietic transition in E8.5 concepti", "created": "Nov.12, 2014", "summary": "During development a specialised subset of endothelial cells, the haemogenic endothelium, undergo an endothelial-to-haematopoietic transition. This process critically involves the transcription factor Runx1. Here we have isolated a specific subpopulation of endothelial cells using a Runx1 enhancer-reporter transgenic mouse line (23GFP). We have compared the gene expression profile of this population to non-23GFP expressing endothelial cells and CD41 expressing haematopoietic progenitor cells to assess whether 23GFP expression marks a biologically distinct subset of endothelium. We used affymetrix microarrays to detail the global programme of gene expression underlying the different populations and identified a distinct expression profile of the 23GFP expressing endothelium. 23GFP transgenic mouse embryos were dissected free of the ectoplacental cone at embryonic day E8.5, pooled, and sorted into 23GFP- and 23GFP+ endothelial (VE-Cad+ Ter119- CD45- CD41-) and CD41+ haematopoietic cells (VE-Cad+ Ter119-CD45-CD41+23GFP+) cells by FACS. Triplicate samples for each cell population were analysed. cDNA synthesis, fragmentation, and hybridisation were performed by the Stanford Protein and Nucleic Acid Facility.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52075", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52075/samples/"}