{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}, {"GSM1253495": {"TIME": "after 20 min bout of exercise"}}, {"GSM1253494": {"TIME": "before exercise"}}], "id": 2123, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes...", "geo_gse_id": "E-GEOD-51835", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 24, "tags": ["cell", "monocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "effects-of-exercise-on-gene-expression-level-in-hu", "geo_id_plat": "E-GEOD-51835_A-AFFY-44", "name": "Effects of exercise on gene expression level in human monocytes", "created": "Jul.11, 2014", "summary": "We tested the hypothesis on the mechanisms responsible for the early control of monocytes function by identifying a discrete set of genes in circulating monocytes that were altered by exercise. Exercise leads to a rapid change in the profile of gene expression in monocytes. Twelve healthy men (22-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 82% of VO2max. A baseline blood sample was taken before the and immediately after the exercise.  Monocytes  were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Monocyte Isolation Kit II human  #130-091-153 and the autoMACS\u00ae Pro Separator). Total RNA was extracted using TRIzol\u00ae.  For this study we used Affymetrix plus 2  (total of 24 chips).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51835", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51835/samples/"}