{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1253170": {"DEVELOPMENTAL STAGE": "17 wk"}}, {"GSM1253170": {"DEVELOPMENTAL STAGE": "17 wk"}}, {"GSM1253170": {"DEVELOPMENTAL STAGE": "17 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}, {"GSM1253167": {"DEVELOPMENTAL STAGE": "6 wk"}}], "id": 2187, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Joint injury and osteoarthritis affect millions of people worldwide, but attempts to  generate articular cartilage using adult...", "geo_gse_id": "E-GEOD-51812", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 15, "tags": ["articular cartilage", "chondrocyte", "joint", "limb", "osteoarthritis", "superficial", "superior", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-51812_A-AFFY-44", "slug": "identification-of-chondrocyte-subsets-during-human", "geo_gds_id": "", "name": "Identification of chondrocyte subsets during human development", "created": "Jul.11, 2014", "summary": "Joint injury and osteoarthritis affect millions of people worldwide, but attempts to  generate articular cartilage using adult stem/progenitor cells have been unsuccessful.  We hypothesized that recapitulation of the human developmental chondrogenic  program using pluripotent stem cells (PSCs) may represent a superior approach for  cartilage restoration. Using laser capture microdissection followed by microarray  analysis, we first defined a surface phenotype  (CD146low/negCD166low/negCD73+CD44lowBMPR1B+) distinguishing the earliest  cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development; pellet  assays confirmed these cells as functional, chondrocyte-restricted progenitors. Flow  cytometry, qPCR and immunohistochemistry at 17 weeks revealed that the superficial  layer of peri-articular chondrocytes was enriched in cells with this surface phenotype.  Isolation of cells with a similar immunophenotype from differentiating human PSCs  revealed a population of CD166negBMPR1B+ putative pre-chondrocytes. Functional  characterization confirmed these cells as cartilage-committed, chondrocyte  progenitors. The identification of a specific molecular signature for primary cartilagecommitted  progenitors may provide essential knowledge for the generation of purified,  clinically relevant cartilage cells from PSCs. A total of 15 samples were analyzed.  In the first comparison, there were 6 biological replicates for both the chondrogenic condensations and total limb cells.  In the second comparison, three biological replicates of chondrocytes from the articular region were compared to the 6 replicates of the condensations.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51812", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51812/samples/"}