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Home › Dataset Library › Identification of chondrocyte subsets during human development

Dataset: Identification of chondrocyte subsets during human development

Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult...

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Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD146low/negCD166low/negCD73+CD44lowBMPR1B+) distinguishing the earliest cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development; pellet assays confirmed these cells as functional, chondrocyte-restricted progenitors. Flow cytometry, qPCR and immunohistochemistry at 17 weeks revealed that the superficial layer of peri-articular chondrocytes was enriched in cells with this surface phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166negBMPR1B+ putative pre-chondrocytes. Functional characterization confirmed these cells as cartilage-committed, chondrocyte progenitors. The identification of a specific molecular signature for primary cartilagecommitted progenitors may provide essential knowledge for the generation of purified, clinically relevant cartilage cells from PSCs. A total of 15 samples were analyzed. In the first comparison, there were 6 biological replicates for both the chondrogenic condensations and total limb cells. In the second comparison, three biological replicates of chondrocytes from the articular region were compared to the 6 replicates of the condensations.

Species:
human

Samples:
15

Source:
E-GEOD-51812

Updated:
Dec.12, 2014

Registered:
Jul.11, 2014


Factors: (via ArrayExpress)
Sample DEVELOPMENTAL STAGE
GSM1253170 17 wk
GSM1253170 17 wk
GSM1253170 17 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk
GSM1253167 6 wk

Tags

  • articular cartilage
  • chondrocyte
  • joint
  • limb
  • osteoarthritis
  • superficial
  • superior
  • surface

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