Dataset: Identification of chondrocyte subsets during human development
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult...
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD146low/negCD166low/negCD73+CD44lowBMPR1B+) distinguishing the earliest cartilage committed cells (pre-chondrocytes) at 5-6 weeks of development; pellet assays confirmed these cells as functional, chondrocyte-restricted progenitors. Flow cytometry, qPCR and immunohistochemistry at 17 weeks revealed that the superficial layer of peri-articular chondrocytes was enriched in cells with this surface phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166negBMPR1B+ putative pre-chondrocytes. Functional characterization confirmed these cells as cartilage-committed, chondrocyte progenitors. The identification of a specific molecular signature for primary cartilagecommitted progenitors may provide essential knowledge for the generation of purified, clinically relevant cartilage cells from PSCs. A total of 15 samples were analyzed. In the first comparison, there were 6 biological replicates for both the chondrogenic condensations and total limb cells. In the second comparison, three biological replicates of chondrocytes from the articular region were compared to the 6 replicates of the condensations.
- Species:
- human
- Samples:
- 15
- Source:
- E-GEOD-51812
- Updated:
- Dec.12, 2014
- Registered:
- Jul.11, 2014
Sample | DEVELOPMENTAL STAGE |
---|---|
GSM1253170 | 17 wk |
GSM1253170 | 17 wk |
GSM1253170 | 17 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |
GSM1253167 | 6 wk |