ArrayExpress Uploader02121Bag-1L GARRPR mutant (poZC-Bag1LNmut)DHTBag-1L GARRPR mutant (poZC-Bag1LNmut)DHTBag-1L GARRPR mutant (poZC-Bag1LNmut)DHTBag-1L GARRPR mutant (poZC-Bag1LNmut)EtOHBag-1L GARRPR mutant (poZC-Bag1LNmut)EtOHBag-1L GARRPR mutant (poZC-Bag1LNmut)EtOHBag-1L wild-type (poZC-Bag1Lwt)DHTBag-1L wild-type (poZC-Bag1Lwt)DHTBag-1L wild-type (poZC-Bag1Lwt)DHTBag-1L wild-type (poZC-Bag1Lwt)EtOHBag-1L wild-type (poZC-Bag1Lwt)EtOHBag-1L wild-type (poZC-Bag1Lwt)EtOHempty vector control (poZC)DHTempty vector control (poZC)DHTempty vector control (poZC)DHTempty vector control (poZC)EtOHempty vector control (poZC)EtOHempty vector control (poZC)EtOHarrayexpress_sid4The BF-3 pocket of the androgen receptor (AR) has been identified as an allosteric modulator of the transactivation function of the AR....24523409E-GEOD-51524/profile/8773/arrayexpressuploader218amino acidandrogencellhormonelineDec.12, 2014Falselncap-prostate-cancer-cell-lines-overexpressing-wiE-GEOD-51524_A-AFFY-44LNCaP prostate cancer cell lines overexpressing wild-type or GARRPR-mutant Bag-1LJul.11, 2014The BF-3 pocket of the androgen receptor (AR) has been identified as an allosteric modulator of the transactivation function of the AR. We now demonstrate that a duplicated GARRPR motif at the N-terminus of the cochaperone Bag-1L functions through this BF-3 domain. Amino acid exchanges in these two motifs impair binding of Bag-1L to the AR but increase the androgen-dependent activation of a subset of AR-target genes. We have therefore identified GARRPR as a novel BF-3 regulatory sequence important for fine-tuning the activity of the receptor. LNCaP cells stably expressing the empty vector construct (=control), or wild-type or N-terminal GARRPR mutant Bag-1L were cultured under hormone-starvation conditions for 72 h and then treated with vehicle or 10 nM DHT for 4 h. Biological triplicate samples were analyzed for each cell line.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51524humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51524/samples/