{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2121, "factors": [{"GSM1246902": {"TRANSFECTION": "Bag-1L GARRPR mutant (poZC-Bag1LNmut)", "TREATMENT": "DHT"}}, {"GSM1246902": {"TRANSFECTION": "Bag-1L GARRPR mutant (poZC-Bag1LNmut)", "TREATMENT": "DHT"}}, {"GSM1246902": {"TRANSFECTION": "Bag-1L GARRPR mutant (poZC-Bag1LNmut)", "TREATMENT": "DHT"}}, {"GSM1246899": {"TRANSFECTION": "Bag-1L GARRPR mutant (poZC-Bag1LNmut)", "TREATMENT": "EtOH"}}, {"GSM1246899": {"TRANSFECTION": "Bag-1L GARRPR mutant (poZC-Bag1LNmut)", "TREATMENT": "EtOH"}}, {"GSM1246899": {"TRANSFECTION": "Bag-1L GARRPR mutant (poZC-Bag1LNmut)", "TREATMENT": "EtOH"}}, {"GSM1246896": {"TRANSFECTION": "Bag-1L wild-type (poZC-Bag1Lwt)", "TREATMENT": "DHT"}}, {"GSM1246896": {"TRANSFECTION": "Bag-1L wild-type (poZC-Bag1Lwt)", "TREATMENT": "DHT"}}, {"GSM1246896": {"TRANSFECTION": "Bag-1L wild-type (poZC-Bag1Lwt)", "TREATMENT": "DHT"}}, {"GSM1246893": {"TRANSFECTION": "Bag-1L wild-type (poZC-Bag1Lwt)", "TREATMENT": "EtOH"}}, {"GSM1246893": {"TRANSFECTION": "Bag-1L wild-type (poZC-Bag1Lwt)", "TREATMENT": "EtOH"}}, {"GSM1246893": {"TRANSFECTION": "Bag-1L wild-type (poZC-Bag1Lwt)", "TREATMENT": "EtOH"}}, {"GSM1246890": {"TRANSFECTION": "empty vector control (poZC)", "TREATMENT": "DHT"}}, {"GSM1246890": {"TRANSFECTION": "empty vector control (poZC)", "TREATMENT": "DHT"}}, {"GSM1246890": {"TRANSFECTION": "empty vector control (poZC)", "TREATMENT": "DHT"}}, {"GSM1246887": {"TRANSFECTION": "empty vector control (poZC)", "TREATMENT": "EtOH"}}, {"GSM1246887": {"TRANSFECTION": "empty vector control (poZC)", "TREATMENT": "EtOH"}}, {"GSM1246887": {"TRANSFECTION": "empty vector control (poZC)", "TREATMENT": "EtOH"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The BF-3 pocket of the androgen receptor (AR) has been identified as an allosteric modulator of the transactivation function of the AR....", "pubmed_id": 24523409, "geo_gse_id": "E-GEOD-51524", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 18, "tags": ["amino acid", "androgen", "cell", "hormone", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "lncap-prostate-cancer-cell-lines-overexpressing-wi", "geo_id_plat": "E-GEOD-51524_A-AFFY-44", "name": "LNCaP prostate cancer cell lines overexpressing wild-type or GARRPR-mutant Bag-1L", "created": "Jul.11, 2014", "summary": "The BF-3 pocket of the androgen receptor (AR) has been identified as an allosteric modulator of the transactivation function of the AR. We now demonstrate that a duplicated GARRPR motif at the N-terminus of the cochaperone Bag-1L functions through this BF-3 domain. Amino acid exchanges in these two motifs impair binding of Bag-1L to the AR but increase the androgen-dependent activation of a subset of AR-target genes. We have therefore identified GARRPR as a novel BF-3 regulatory sequence important for fine-tuning the activity of the receptor. LNCaP cells stably expressing the empty vector construct (=control), or wild-type or N-terminal GARRPR mutant Bag-1L were cultured under hormone-starvation conditions for 72 h and then treated with vehicle or 10 nM DHT for 4 h. Biological triplicate samples were analyzed for each cell line.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51524", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51524/samples/"}