ArrayExpress Uploader07462not specifiednot specifiednot specifiednot specifiednot specifiednot specifiedE7.5Mouse whole embryo tissues at E7.5E7.5Mouse whole embryo tissues at E7.5E7.5Mouse whole embryo tissues at E7.5E8.5Whole heart tube tissuesE8.5Whole heart tube tissuesE8.5Whole heart tube tissuesE9.5Left ventricle tissuesE9.5Left ventricle tissuesE9.5Left ventricle tissuesE9.5Right ventricle tissuesE9.5Right ventricle tissuesE9.5Right ventricle tissuesE12.5Left ventricle tissuesE12.5Left ventricle tissuesE12.5Left ventricle tissuesE12.5Right ventricle tissuesE12.5Right ventricle tissuesE12.5Right ventricle tissuesE14.5Left ventricle tissuesE14.5Left ventricle tissuesE14.5Left ventricle tissuesE14.5Right ventricle tissuesE14.5Right ventricle tissuesE14.5Right ventricle tissuesE18.5Left ventricle tissuesE18.5Left ventricle tissuesE18.5Left ventricle tissuesE18.5Right ventricle tissuesE18.5Right ventricle tissuesE18.5Right ventricle tissues3 days after birthLeft ventricle tissues3 days after birthLeft ventricle tissues3 days after birthLeft ventricle tissues3 days after birthRight ventricle tissues3 days after birthRight ventricle tissues3 days after birthRight ventricle tissuesadultLeft ventricle tissueadultLeft ventricle tissueadultLeft ventricle tissueadultRight ventricle tissueadultRight ventricle tissueadultRight ventricle tissuearrayexpress_sid6Mammalian heart development is built on highly conserved molecular mechanisms with polygenetic perturbations resulting in a spectrum of...24803680E-GEOD-51483/profile/8773/arrayexpressuploader245atlascellcongenital heart diseasediseasegenomeheartheart diseaseleftlinenotchpointrightright ventriclestem cellventricleDec.12, 2014Falsetranscriptional-atlas-of-cardiogenesis-maps-congenE-GEOD-51483_A-AFFY-45Transcriptional Atlas of Cardiogenesis Maps Congenital Heart Disease InteractomeNov.12, 2014Mammalian heart development is built on highly conserved molecular mechanisms with polygenetic perturbations resulting in a spectrum of congenital heart diseases (CHD). However, the transcriptional landscape of cardiogenic ontogeny that regulates proper cardiogenesis remains largely based on candidate-gene approaches. Herein, we designed a time-course transcriptome analysis to investigate the genome-wide expression profile of innate murine cardiogenesis ranging from embryonic stem cells to adult cardiac structures. This comprehensive analysis generated temporal and spatial expression profiles, prioritized stage-specific gene functions, and mapped the dynamic transcriptome of cardiogenesis to curated pathways. Reconciling the bioinformatics of the congenital heart disease interactome, we deconstructed disease-centric regulatory networks encoded within this cardiogenic atlas to reveal stage-specific developmental disturbances clustered on epithelial-to-mesenchymal transition (EMT), BMP regulation, NF-AT signaling, TGFb-dependent induction, and Notch signaling. Therefore, this cardiogenic transcriptional landscape defines the time-dependent expression of cardiac ontogeny and prioritizes regulatory networks at the interface between health and disease. To interrogate the temporal and spatial expression profiles across the entire genome during mammalian heart development, we designed a time-course microarray experiment using the mouse model at defined stages of cardiogenesis, starting with embryonic stem cells (ESC, R1 stem cell line), early embryonic developmental stages: E7.5 whole embryos, E8.5 heart tubes, left and right ventricle tissues at E9.5, E12.5, E14.5, E18.5 to 3 days after birth (D3) and adult heart (Figure 1A). At each time point, microarray experiments were performed on triplicate biological samples. Starting at E9.5, tissue samples from left ventricles (LV) and right ventricles (RV) were microdissected for RNA purification and microarray analysis to determine spatially differential gene expression between LV and RV during heart development.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51483mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51483/samples/