{"platform": 6, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM1245603 1": {}}, {"GSM1245604 1": {}}, {"GSM1245605 1": {}}, {"GSM1245606 1": {}}, {"GSM1245607 1": {}}, {"GSM1245608 1": {}}, {"GSM1245609 1": {}}, {"GSM1245610 1": {}}, {"GSM1245611 1": {}}, {"GSM1245612 1": {}}, {"GSM1245613 1": {}}, {"GSM1245614 1": {}}, {"GSM1245615 1": {}}, {"GSM1245616 1": {}}, {"GSM1245617 1": {}}], "id": 7461, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51432", "summary_wrapped": "To evaluate the effect of \u03b2-cryptoxanthin on diet-induced NASH, we fed a high-cholesterol and high-fat diet (CL diet) with or without...", "pubmed_id": 24858832, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 15, "tags": ["cell", "crf", "lipid", "lipopolysaccharide", "stellate"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "cryptoxanthin-suppresses-inflammatory-gene-expres", "geo_id_plat": "E-GEOD-51432_A-AFFY-45", "name": "\u03b2-Cryptoxanthin suppresses inflammatory gene expression in diet-induced nonalcoholic steatohepatitis in mice", "created": "Nov.12, 2014", "summary": "To evaluate the effect of \u03b2-cryptoxanthin on diet-induced NASH, we fed a high-cholesterol and high-fat diet (CL diet) with or without 0.003% \u03b2-cryptoxanthin to C56BL/6J mice for 12 weeks. After feeding, \u03b2-cryptoxanthin attenuated fat accumulation, increases in Kupffer and activated stellate cells, and fibrosis in CL diet-induced NASH in the mice. Comprehensive gene expression analysis showed that although \u03b2-cryptoxanthin histochemically reduced steatosis, it was more effective in inhibiting inflammatory gene expression change in NASH. \u03b2-Cryptoxanthin reduced the alteration of expression of genes associated with cell death, inflammatory responses, infiltration and activation of macrophages and other leukocytes, quantity of T cells, and free radical scavenging. However, it showed little effect on the expression of genes related to cholesterol and other lipid metabolism. The expression of markers of M1 and M2 macrophages, T helper cells, and cytotoxic T cells was significantly induced in NASH and reduced by \u03b2-cryptoxanthin. \u03b2-Cryptoxanthin suppressed the expression of lipopolysaccharide (LPS)-inducible and/or TNF\u03b1-inducible genes and the antioxidant enzyme glutathione peroxidase 1 in NASH. Thus, \u03b2-cryptoxanthin suppresses inflammation and the resulting fibrosis probably by primarily suppressing the increase and activation of macrophages and other immune cells. Reducing oxidative stress is likely to be a major mechanism of inflammation and injury suppression in the livers of mice with NASH. Eight-week old male C57BL/6J mice were fed for 12 weeks on a CRF-1 standard chow (control), a high-cholesterol and high-fat diet (CL diet; 38.23% CRF-1, 60% cocoa butter, 1.25% cholesterol, 0.5% sodium cholate) or a CL diet containing 0.003% \u03b2-cryptoxanthin.", "geo_gse_id": "E-GEOD-51432", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51432/samples/"}