{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1237668": {"CELL TYPE": "non-pigmented melanocytes", "GENOTYPE": "homozygous point mutation in tyrosinase gene"}}, {"GSM1237668": {"CELL TYPE": "non-pigmented melanocytes", "GENOTYPE": "homozygous point mutation in tyrosinase gene"}}, {"GSM1237670": {"CELL TYPE": "pigmented melanocytes", "GENOTYPE": "wild type (point mutation corrected)"}}, {"GSM1237670": {"CELL TYPE": "pigmented melanocytes", "GENOTYPE": "wild type (point mutation corrected)"}}], "id": 7438, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Immortalized, amelanotic melanocytes isolted from skin of Balb/c express enzymatically-inactive tyrosinase due to a homozygous point...", "geo_gse_id": "E-GEOD-51073", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cell", "line", "melanin", "melanocyte", "point", "skin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-non-pigmented-and-pigmented-m", "geo_id_plat": "E-GEOD-51073_A-AFFY-45", "name": "Expression data from non-pigmented and pigmented mouse melanocytes", "created": "Nov.12, 2014", "summary": "Immortalized, amelanotic melanocytes isolted from skin of Balb/c express enzymatically-inactive tyrosinase due to a homozygous point mutation (TGT->TCT) in tyrosinase gene, resulting in a lack of melanin . To serve as a control cell line, pigmentation was restored in these cells by correcting the point mutation using an RNA-DNA oligonucleotide (kingly gift from Dr. Alexeev  Y. Vitali). We used microarray to detail the effect of tyrosinase mutation on gene expression in normal versus mutant melanocytes. Pigmented and non-pigmented melanocytes were grown for RNA extraction and hybridization on Affymetrix microarrays. We used both normal and mutant melanocyte in order to obtain expression profiles. We then examined variances in gene expression between the two cell lines.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51073", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-51073/samples/"}