{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1230365 1": {}}, {"GSM1230366 1": {}}, {"GSM1230367 1": {}}, {"GSM1230368 1": {}}, {"GSM1230369 1": {}}, {"GSM1230370 1": {}}, {"GSM1230371 1": {}}, {"GSM1230372 1": {}}, {"GSM1230373 1": {}}, {"GSM1230374 1": {}}, {"GSM1230375 1": {}}, {"GSM1230376 1": {}}], "id": 7429, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The \u03b2 subunits of voltage-gated calcium channels regulate surface expression and gating of CaV1 and CaV2 \u03b11 subunits, and thus contribute...", "geo_gse_id": "E-GEOD-50822", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["axon", "cerebellum", "distal", "genome", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "differential-neuronal-targeting-of-a-new-and-2-kno", "geo_id_plat": "E-GEOD-50822_A-AFFY-45", "name": "Differential neuronal targeting of a new and 2 known calcium channel \u03b24 subunit splice variants correlates with their regulation of gene expression", "created": "Nov.12, 2014", "summary": "The \u03b2 subunits of voltage-gated calcium channels regulate surface expression and gating of CaV1 and CaV2 \u03b11 subunits, and thus contribute to neuronal excitability, neurotransmitter release and calcium-induced gene regulation. In addition certain \u03b2 subunits are targeted into the nucleus, where they directly interact with the epigenetic machinery. Whereas their involvement in this multitude of functions is reflected by a great molecular heterogeneity of \u03b2 isoforms derived from four genes and abundant alternative splicing, little is known about the roles of individual \u03b2 variants in specific neuronal functions. In the present study, an alternatively spliced \u03b24 subunit lacking the variable N-terminus (\u03b24e) is identified. It is highly expressed in mouse cerebellum and cultured cerebellar granule cells (CGC) and modulates P/Q-type calcium currents in tsA cells and CaV2.1 surface expression in neurons. Compared to the other two known full-length \u03b24 variants (\u03b24a, \u03b24b) \u03b24e is most abundantly expressed in the distal axon, but lacks nuclear targeting properties. To examine the importance of nuclear targeting of \u03b24 subunits for transcriptional regulation, we performed whole genome expression profiling of CGCs from lethargic mice individually reconstituted with \u03b24a, \u03b24b, and \u03b24e. Notably, the number of genes regulated by each \u03b24 splice variant correlated with the rank order of their nuclear targeting properties (\u03b24b> \u03b24a> \u03b24e). Together these findings support isoform-specific functions of \u03b24 splice variant in neurons, with \u03b24b playing a dual role in channel modulation and gene regulation, while the newly detected \u03b24e variant serves exclusively in calcium channel-dependent functions. We used microarrays to identify gene expression changes caused by \u03b24 splice variants (\u03b24a, \u03b24b and \u03b24e) of the voltage gated calcium channel in cultured cerebellar granule cells of lethargic mice Cultured cerebellar granule cells from lethargic (129/SvJ background) mice reconstituted with the \u03b24 splice variants (\u03b24a, \u03b24b and \u03b24e) were compared to eGFP transfected controls", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50822", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50822/samples/"}