{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1227378 1": {}}, {"GSM1227379 1": {}}, {"GSM1227380 1": {}}, {"GSM1227381 1": {}}, {"GSM1227382 1": {}}, {"GSM1227383 1": {}}], "id": 7425, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Following antigen encounter by CD4 T cells, polarizing cytokines induce the expression of master regulators that control differentiation....", "geo_gse_id": "E-GEOD-50729", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["allergic asthma", "asthma", "cell", "cytokine", "histone", "lysine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-polycomb-protein-ezh2-regulates-differentiatio", "geo_id_plat": "E-GEOD-50729_A-AFFY-45", "name": "The polycomb protein Ezh2 regulates differentiation and plasticity of CD4 T helper type-1 and type-2 cells.", "created": "Nov.12, 2014", "summary": "Following antigen encounter by CD4 T cells, polarizing cytokines induce the expression of master regulators that control differentiation.  Inactivation of the histone methyltransferase Ezh2 was found to specifically enhance T-helper (Th)1 and Th2 cell differentiation and plasticity. Ezh2 directly bound and facilitated correct expression of Tbx21 and Gata3 in differentiating Th1 and Th2 cells, accompanied by substantial tri-methylation at lysine 27 of histone 3 (H3K27-Me3). In addition, Ezh2 deficiency resulted in spontaneous generation of discrete IFN-\u03b3 and Th2 cytokine-producing populations in non-polarizing cultures, and under these conditions IFN-\u03b3  expression was largely dependent on enhanced expression of the transcription factor Eomesodermin. In vivo, Loss of Ezh2 caused increased pathology in a model of allergic asthma and resulted in progressive accumulation of memory phenotype Th2 cells.  This study establishes a functional link between Ezh2 and transcriptional regulation of lineage-specifying genes in terminally differentiated CD4 T cells. Wild type and Ezh2 knock out unpolarized Th cells, Th1 cells and Th2 cells are profiled for mRNA expression", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50729", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50729/samples/"}