ArrayExpress Uploaderarrayexpress_sidmousecontrolcontrolcontrolcontrolcontrolcontrolL-prolineL-prolineL-prolineL-prolineL-prolineL-proline742306We found that the non-essential amino acid L-Proline (L-Pro) acts as a signaling molecule that promotes the conversion of embryonic stem...E-GEOD-50603/profile/8773/arrayexpressuploader112amino acidcellembryonic stem cellgenomeprolinespindlestem cellDec.12, 2014Falseeffect-of-l-proline-on-mouse-embryonic-stem-cellsE-GEOD-50603_A-AFFY-45Effect of L-Proline on mouse embryonic stem cells (ESCs)Nov.12, 2014We found that the non-essential amino acid L-Proline (L-Pro) acts as a signaling molecule that promotes the conversion of embryonic stem cells (ESCs) into mesenchymal-like, spindle-shaped, highly motile, invasive pluripotent stem cells. This embryonic stem cell-to-mesenchymal-like transition (esMT) is accompanied by a genome-wide remodeling of the transcriptome We used microarrays to elucidate whether a diverse transcriptional program is the basis of the morphological and motility differences between ESCs and L- Proline treated ESCs (PiCs) Total RNA was extracted from Control (ESCs) and L-Proline treated mouse embryonic stem cells (PiCs) and hybridized on Affimetrix microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50603http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50603/samples/