{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7419, "factors": [{"GSE5042GSM113461": {"Genotype": "PGC-1alpha -/-", "RNAi": "control siRNA"}}, {"GSE5042GSM113461": {"Genotype": "PGC-1alpha -/-", "RNAi": "control siRNA"}}, {"GSE5042GSM113463": {"Genotype": "PGC-1alpha -/-", "RNAi": "PGC-1beta siRNA knockdown"}}, {"GSE5042GSM113463": {"Genotype": "PGC-1alpha -/-", "RNAi": "PGC-1beta siRNA knockdown"}}, {"GSE5042GSM113409": {"Genotype": "wild_type", "RNAi": "untreated"}}, {"GSE5042GSM113409": {"Genotype": "wild_type", "RNAi": "untreated"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Mitochondria play an essential role in the ability of brown fat to generate heat, and the PGC-1 coactivators control several aspects of...", "pubmed_id": 16679291, "geo_gse_id": "E-GEOD-5042", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 6, "tags": ["adipose tissue", "brown adipose tissue"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-differentiated-brown-ad", "geo_id_plat": "E-GEOD-5042_A-AFFY-45", "name": "Transcription profiling of differentiated brown adipocytes from wild type, PGC-1 alpha knockout, or PGC-1 alpha knockout and PGC-1 beta knockdown mice to investigate the roles of PGC-1 coactivators in brown fat differentiation.", "created": "Nov.12, 2014", "summary": "Mitochondria play an essential role in the ability of brown fat to generate heat, and the PGC-1 coactivators control several aspects of mitochondrial biogenesis. To investigate their specific roles in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1\u00b1. We could then efficiently knockdown PGC-1\u03b2 expression by shRNA expression. Loss of PGC-1\u00b1 did not alter brown fat differentiation but severely reduced the induction of thermogenic genes. Cells deficient in either PGC-1\u03b1 or PGC-1\u03b2 coactivators showed a small decrease in the differentiation-dependant program of mitochondrial biogenesis and respiration; however, this increase in mitochondrial number and function was totally abolished during brown fat differentiation when both PGC-1\u00b1 and PGC-1 were deficient. These data show that PGC-1\u00b1 is essential for brown fat thermogenesis but not brown fat differentiation, and the PGC-1 coactivators play an absolutely essential but complementary function in differentiation-induced mitochondrial biogenesis. Affymetrix microarray analysis of total RNA from wt, PGC-1\u00b1 KO and PGC-1\u00b1 KO; cells expressing an RNAi specific for PGC-1 knockdown was performed. Of the 461; mitochondrial genes analyzed, 181 were found to be at least 20% different between wt; and defective PGC-1\u00b1 and \u03b2 adipocytes (p < 0.05). More than 85% of these genes were downregulated in cells deficient for PGC-1alpha and PGC-1beta. Experiment Overall Design: Brown preadipocytes that were either WT, KO for PGC-1alpha, or KO for PGC-1alpha and deficient for PGC-1beta (knockdown through siRNA expression) were differentiated for seven days. RNA was made from biological replicates of the three different types of brown adipocytes (WT, KO expressing a control siRNA, KO expressing a siRNA specific for PGC-1beta knockdown).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5042", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5042/samples/"}