{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7416, "factors": [{"GSM1218155": {"TREATMENT": "IMO-8400", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218155": {"TREATMENT": "IMO-8400", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218155": {"TREATMENT": "IMO-8400", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218155": {"TREATMENT": "IMO-8400", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218155": {"TREATMENT": "IMO-8400", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218160": {"TREATMENT": "IMO-3100", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218160": {"TREATMENT": "IMO-3100", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218160": {"TREATMENT": "IMO-3100", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218160": {"TREATMENT": "IMO-3100", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218160": {"TREATMENT": "IMO-3100", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218165": {"TREATMENT": "saline", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218165": {"TREATMENT": "saline", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218165": {"TREATMENT": "saline", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218165": {"TREATMENT": "saline", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218165": {"TREATMENT": "saline", "CONDITION": "IL23 induced phenotype"}}, {"GSM1218170": {"TREATMENT": "na\ufffdve", "CONDITION": "normal"}}, {"GSM1218170": {"TREATMENT": "na\ufffdve", "CONDITION": "normal"}}, {"GSM1218170": {"TREATMENT": "na\ufffdve", "CONDITION": "normal"}}, {"GSM1218170": {"TREATMENT": "na\ufffdve", "CONDITION": "normal"}}, {"GSM1218170": {"TREATMENT": "na\ufffdve", "CONDITION": "normal"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Psoriasis is a complex inflammatory disease resulting from the activation of T helper (Th) 1 and Th17 cells.  Recent evidence suggests...", "pubmed_id": 24386404, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 20, "tags": ["disease", "keratin", "psoriasis", "skin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-50400_A-AFFY-45", "slug": "suppression-of-molecular-inflammatory-pathways-by", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50400", "geo_gds_id": "", "name": "Suppression of molecular inflammatory pathways by Toll-like Receptor 7, 8, and 9 antagonists in a model of IL-23-induced skin inflammation", "created": "Nov.12, 2014", "summary": "Psoriasis is a complex inflammatory disease resulting from the activation of T helper (Th) 1 and Th17 cells.  Recent evidence suggests that abnormal activation of Toll-like receptors (TLRs) 7, 8 and 9 contributes to the initiation and maintenance of psoriasis.  We have evaluated the effects of TLR antagonists on the gene expression profile in an IL-23-induced skin inflammation model in mice.  Psoriasis-like skin lesions were induced in C57BL/6 mice by intradermal injection of IL-23 in the dorsum.  Two TLR antagonists were compared: IMO-3100, an antagonist of TLRs 7 and 9, and IMO-8400, an antagonist of TLRs 7, 8 and 9, both of which previously have been shown to reduce epidermal hyperplasia in this model.  Skin gene expression profiles of IL-23-induced inflammation were compared with or without TLR antagonist treatment.  IL-23 injection resulted in alteration of 5100 gene probes (fold change \u2265 2, FDR < 0.05) including IL-17 pathways that are up-regulated in psoriasis vulgaris.   Targeting TLRs 7, 8 and 9 with IMO-8400 resulted in modulation of more than 2300 mRNAs while targeting TLRs 7 and 9 with IMO-3100 resulted in modulation of more than 1900 mRNAs.  Both agents strongly decreased IL-17A expression (>12-fold reduction), normalized IL-17 induced genes such as beta-defensin and CXCL1, and normalized aberrant expression of keratin 16 (indicating epidermal hyperplasia). These results suggest that IL-23-driven inflammation in mouse skin may be dependent on signaling mediated by TLRs 7, 8, and 9 and that these receptors represent novel therapeutic targets in psoriasis vulgaris and other diseases with similar pathophysiology. Expression profiles for  mice with IL23-induced phenotype (psoriasisform) at baseline and after treatment with two doses of TLR7/8/9 antagonist and saline. Samples for nomal mice are also available", "geo_gse_id": "E-GEOD-50400", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50400/samples/"}