ArrayExpress Uploaderarrayexpress_sid74153 wkPitx2 Flox/Flox3 wkPitx2 Flox/Flox3 wkPitx2 Flox/Flox3 wkMCKcre Pitx2 Flox/Flox3 wkMCKcre Pitx2 Flox/Flox3 wkMCKcre Pitx2 Flox/Flox6 wkPitx2 Flox/Flox6 wkPitx2 Flox/Flox6 wkPitx2 Flox/Flox6 wkMCKcre Pitx2 Flox/Flox6 wkMCKcre Pitx2 Flox/Flox6 wkMCKcre Pitx2 Flox/Flox12 wkPitx2 Flox/Flox12 wkPitx2 Flox/Flox12 wkPitx2 Flox/Flox12 wkMCKcre Pitx2 Flox/Flox12 wkMCKcre Pitx2 Flox/Flox12 wkMCKcre Pitx2 Flox/Flox06Pitx2 is the homeobox gene located in proximity to the human 4q25 familial atrial fibrillation locus. Pitx2 haploinsufficient mice are...24395921E-GEOD-50399/profile/8773/arrayexpressuploader218atriumcellcell junctionfamilial atrial fibrillationgenomeheartpointsinusDec.12, 2014FalseE-GEOD-50399_A-AFFY-45gene-expression-data-of-postnatal-pitx2-deficientGene expression data of postnatal Pitx2 deficient heartNov.12, 2014Pitx2 is the homeobox gene located in proximity to the human 4q25 familial atrial fibrillation locus. Pitx2 haploinsufficient mice are prone to pacing induced atrial fibrillation indicating that reduced Pitx2 promotes an arrhythmogenic substrate within the atrium. Here, we inactivated Pitx2 in postnatal heart and discovered that unstressed adult Pitx2 mutant mice had sinus node dysfunction with impaired atrial conduction, an arrhythmia closely associated with atrial fibrillation. A genome-wide search for Pitx2 transcriptional targets using ChIP-sequencing and RNA expression profiling shows that Pitx2 represses target genes encoding cell junction proteins, ion channels, and critical transcriptional regulators many of which have been implicated in human atrial fibrillation by genome wide association studies. Pitx2 control and mutant hearts were collected from 3-, 6- and 12-week-old mice. At each time point, three cotrols and three mutants were collected as biological replicates. cDNA microarray analysis was performed using Affymetrix GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50399mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50399/samples/