{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1215114": {"CELL SUBSET": "Th1Th17 cells", "DONOR": "580"}}, {"GSM1215113": {"CELL SUBSET": "Th1 cells", "DONOR": "580"}}, {"GSM1215112": {"CELL SUBSET": "Th1Th17 cells", "DONOR": "674"}}, {"GSM1215": {"CELL SUBSET": "Th1 cells", "DONOR": "674"}}, {"GSM1215110": {"CELL SUBSET": "Th1Th17 cells", "DONOR": "676"}}, {"GSM1215109": {"CELL SUBSET": "Th1 cells", "DONOR": "676"}}, {"GSM1215108": {"CELL SUBSET": "Th1Th17 cells", "DONOR": "661"}}, {"GSM1215107": {"CELL SUBSET": "Th1 cells", "DONOR": "661"}}], "id": 2311, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We previously demonstrated that Th1Th17 cells are highly permissive to HIV-1, whereas Th1 cells are relatively resistant. Here, we...", "geo_gse_id": "E-GEOD-50175", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["genome", "superior"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-human-th1-and-th1th17-cells", "geo_id_plat": "E-GEOD-50175_A-AFFY-44", "name": "Expression data from human Th1 and Th1Th17 cells", "created": "Jul.12, 2014", "summary": "We previously demonstrated that Th1Th17 cells are highly permissive to HIV-1, whereas Th1 cells are relatively resistant. Here, we investigated molecular mechanisms underlying these differences. Superior HIV replication in Th1Th17 vs. Th1 cells was regulated by entry and post-entry mechanisms. We used microarrays to detail the gene expression signatures caracterizing Th1 cells from Th1Th17. Primary human Th1 (CXCR3+CCR6- phenotype) and Th1Th17 (CXCR3+CCR6- phenotype) CD4+ T-cells were isolated by flow cytometry from HIV-uninfected, healthy donors. Cells were stimulated via CD3/CD28 for 3 days. The RNA was extracted and hybridized on the GeneChip\u00ae Human Genome U133 Plus 2.0 Array (Affymetrix).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50175", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50175/samples/"}