{"platform": 3, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM109204 1": {"COMPOUND": "ethanol", "TIME": 0.5}}, {"GSM109205 1": {"COMPOUND": "dexamethasone", "TIME": 0.5}}, {"GSM109206 1": {"COMPOUND": "ethanol", "TIME": 2.0}}, {"GSM109207 1": {"COMPOUND": "dexamethasone", "TIME": 2.0}}, {"GSM109208 1": {"COMPOUND": "ethanol", "TIME": 4.0}}, {"GSM109209 1": {"COMPOUND": "dexamethasone", "TIME": 4.0}}, {"GSM109210 1": {"COMPOUND": "ethanol", "TIME": 24.0}}, {"GSM109211 1": {"COMPOUND": "dexamethasone", "TIME": 24.0}}, {"GSM109212 1": {"COMPOUND": "ethanol", "TIME": 0.5}}, {"GSM109213 1": {"COMPOUND": "dexamethasone", "TIME": 0.5}}, {"GSM109214 1": {"COMPOUND": "ethanol", "TIME": 2.0}}, {"GSM109215 1": {"COMPOUND": "dexamethasone", "TIME": 2.0}}, {"GSM109216 1": {"COMPOUND": "ethanol", "TIME": 4.0}}, {"GSM109217 1": {"COMPOUND": "dexamethasone", "TIME": 4.0}}, {"GSM109218 1": {"COMPOUND": "ethanol", "TIME": 24.0}}, {"GSM109219 1": {"COMPOUND": "dexamethasone", "TIME": 24.0}}, {"GSM109220 1": {"COMPOUND": "ethanol", "TIME": 0.5}}, {"GSM109221 1": {"COMPOUND": "dexamethasone", "TIME": 0.5}}, {"GSM109222 1": {"COMPOUND": "ethanol", "TIME": 2.0}}, {"GSM109223 1": {"COMPOUND": "dexamethasone", "TIME": 2.0}}, {"GSM109224 1": {"COMPOUND": "ethanol", "TIME": 4.0}}, {"GSM109225 1": {"COMPOUND": "dexamethasone", "TIME": 4.0}}, {"GSM109226 1": {"COMPOUND": "ethanol", "TIME": 24.0}}, {"GSM109227 1": {"COMPOUND": "dexamethasone", "TIME": 24.0}}], "id": 9875, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-4917", "summary_wrapped": "This series contain time course microarray data from MCF10A-Myc cells treated with either ethanol or Dexamethasone for 30 min, 2 hr, 4...", "pubmed_id": 16690749, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 24, "tags": ["genome"], "lastmodified": "Jan.20, 2015", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-mcf10a-myc-breast", "geo_id_plat": "E-GEOD-4917_A-AFFY-33", "name": "Transcription profiling of human MCF10A-Myc breast cancer cell line treated with dexamethazone or an ethanol control, sampled at 4 timepoints", "created": "Jan.20, 2015", "summary": "This series contain time course microarray data from MCF10A-Myc cells treated with either ethanol or Dexamethasone for 30 min, 2 hr, 4 hr, and 24 hr. This series contains three biological replicates that were analyzed as independent replicate experiments (data were normalized within each replicate experiment, not across all samples). Keywords: time course MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with either vehicle (ethanol) or Dex (10-6 M) for 0.5, 2, 4 or 24 hrs.  Total RNA from each sample was extracted using Qiagen\u2019s RNeasy Kit.  Preparation of biotinylated cRNA and hybridization to oligonucleotide arrays (Affymetrix human genome genechip HG-U133A) were performed at the University of Chicago Microarray Core Facility.  HG-U133A genechip contains 22,215 probe sets that represent approximately 16,000 human genes.  The complete procedure was repeated independently three times.  Gene chips were scanned and analyzed using Robust Multi-array Average (RMA) algorithm.", "geo_gse_id": "E-GEOD-4917", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-4917/samples/"}