{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM1191944": {"STABLE TRANSFECTION": "vector control"}}, {"GSM1191944": {"STABLE TRANSFECTION": "vector control"}}, {"GSM1191944": {"STABLE TRANSFECTION": "vector control"}}, {"GSM119194": {"STABLE TRANSFECTION": "TAGLN"}}, {"GSM119194": {"STABLE TRANSFECTION": "TAGLN"}}, {"GSM119194": {"STABLE TRANSFECTION": "TAGLN"}}], "id": 2350, "pop_total": 0, "platform": 4, "summary_wrapped": "[u'Transgelin was the top-ranked marker of metastatic potential identified in the comparison of node-positive colorectal cancer (CRC)...", "geo_gse_id": "E-GEOD-48998", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cancer", "colorectal cancer"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-of-tagln-overexpressing-rko-human", "geo_id_plat": "E-GEOD-48998_A-AFFY-44", "name": "Expression data of TAGLN-overexpressing RKO human colon cancer cells", "created": "Jul.12, 2014", "summary": "[u'Transgelin was the top-ranked marker of metastatic potential identified in the comparison of node-positive colorectal cancer (CRC) versus node-negative CRC in our previous study. Transgelin is localized in the nucleus of cultured CRC cells and microRNA-mediated knockdown of TAGLN (the gene encoding transgelin) expression modulates the expression of genes involved in the epithelial-to-mesenchymal transition. We performed gene expression profiling on control and transgelin-overexpressing RKO cells using Affymetrix microarray technology. The plasmid carrying TAGLN was generated using the pcDNA6.2/EmGFP-Bsd/V5-DEST vector (Invitrogen, Carlsbad, CA) and the pDONR221-TAGLN-mut plasmid from a previous study by an LR recombination reaction (', {u'a': {u'href': u'http://www.invitrogen.com', u'target': u'_blank', u'$': u'http://www.invitrogen.com'}}, u'). After lipofectamine-mediated transfection of the TAGLN-carrying plasmid or the vector-only control plasmid, stable transfectants were selected and cultured in medium containing 25 \\u03bcg/ml blasticidin followed by RNA extraction and hybridization on Affymetrix microarrays. Six samples were obtained from three independent experiments (technical replicates).']", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48998", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48998/samples/"}