ArrayExpress Uploader0human184B5HMEC-derived culture adapted but not transformed samplesculture adapted184A1HMEC-derived culture adapted but not transformed samplesculture adaptedHMLE-PRHMEC-derived culture adapted but not transformed samplesculture adaptedHMLEHMEC-derived culture adapted but not transformed samplesculture adaptedMDA-MB-231established human breast cancer cell linesculture adaptedBT549established human breast cancer cell linesculture adaptedBT483established human breast cancer cell linesculture adaptedBT20established human breast cancer cell linesculture adaptedBT474established human breast cancer cell linesculture adaptedT74Destablished human breast cancer cell linesculture adaptedZR_75-30established human breast cancer cell linesculture adaptedMCF7established human breast cancer cell linesculture adaptedHCC3153established human breast cancer cell linesculture adaptedHCC1187established human breast cancer cell linesculture adaptednot specifiedbreast tumor cells from pleural effusion samples, uncultureddirectly purified, unculturednot specifiedbreast tumor cells from pleural effusion samples, uncultureddirectly purified, unculturednot specifiedbreast tumor cells from pleural effusion samples, uncultureddirectly purified, unculturednot specifiedpurified normal mammary stromal cellsdirectly purified, unculturednot specifiedpurified normal mammary stromal cellsdirectly purified, unculturednot specifiedpurified normal mammary stromal cellsdirectly purified, unculturednot specifiedpurified normal CD10+ mammary epithelial cellsdirectly purified, unculturednot specifiedpurified normal BerEp4+ mammary epithelial cellsdirectly purified, unculturednot specifiedpurified normal BerEp4+ mammary epithelial cellsdirectly purified, unculturednot specifiedpurified normal CD10+ mammary epithelial cellsdirectly purified, unculturednot specifiedpurified normal BerEp4+ mammary epithelial cellsdirectly purified, unculturednot specifiedpurified normal CD10+ mammary epithelial cellsdirectly purified, uncultured1209arrayexpress_sid3A small number of tumor-derived cell lines have formed the mainstay of cancer therapeutic development, yielding drugs with impact...E-GEOD-48984/profile/8773/arrayexpressuploader326breastbreast cancercancercelldiseaseepithelial cellglutaminestromal cellDec.12, 2014Falseglutamine-sensitivity-analysis-identifies-the-xctE-GEOD-48984_A-AFFY-33Glutamine sensitivity analysis identifies the xCT antiporter as a common triple negative breast tumor therapeutic target.Jun.18, 2014A small number of tumor-derived cell lines have formed the mainstay of cancer therapeutic development, yielding drugs with impact typically measured as months to disease progression. To develop more effective breast cancer therapeutics, and more readily understand their potential clinical impact, we constructed a functional metabolic portrait of 46 independently-derived breast tumorigenic cell lines, contrasted with purified normal breast epithelial subsets, freshly isolated pleural effusion breast tumor samples and culture-adapted, non-tumorigenic mammary epithelial cell derivatives. We report our analysis of glutamine uptake, dependence, and identification of a significant subset of triple negative samples that are glutamine auxotrophs. This NCBI GEO submission comprises a small datasest generated to compare the expression profiles of the above nontumorigenic, purified normal and purified pleural effusion samples with 10 established breast cancer-derived cell lines. This dataset was subsequently merged with a previously published expression dataset derived from 45 independent breast cancer derived cell lines (Neve, et al 2006), and analyses contrasting various subsets of the merged dataset were published. Expression data from 26 samples, no replicates: purified normal human mammary epithelial breast cellular subsets CD10+, BerEP4+, and remaining stromal cell samples from 3 independent anonymous donors; 3 anonymous purified human breast cancer pleural effusion samples; 4 HMEC-derived culture adapted but not transformed samples (184A1, 184B5, HMLE, HMLE-PR); and 10 established human breast cancer cell lines.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48984http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48984/samples/