ArrayExpress Uploader0humanMDA-MB231 breast cancer cellsMDA-MB231 breast cancer cellsMDA-MB231 breast cancer cellsnone (control)none (control)none (control)2170arrayexpress_sid4We studied the crosstalk between tumor and endothelial cells to explore the role of tumor microenvironment on cancer growth and...E-GEOD-48786/profile/8773/arrayexpressuploader16breastbreast tumorcancercytokineendotheliumserumDec.12, 2014Falseexpression-data-from-endothelial-cells-sorted-fromE-GEOD-48786_A-AFFY-44Expression data from endothelial cells sorted from breast cancer cells MDA-MB231 as compared with normal endothelial cellsJul.11, 2014We studied the crosstalk between tumor and endothelial cells to explore the role of tumor microenvironment on cancer growth and progression. As part of our investigation, we showed that contact-dependent interaction of endothelial cells with breast tumor cells triggered the differential expression of a large number of genes in endothelium. Endothelial and MDA-MB231 cells were co-cultured together under serum- cytokine-free environment for 5 days followed by sorting endothelial cells and extracting RNA for microarray analysis. All conditions were made in triplicate. Endothelial cells were obtained using the methods described in Seandel M. & Butler J.M., 2008. WCMC-Q Genomics Corehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48786http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48786/samples/