{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1183862": {"AGE": "1 year", "STIMULATED WITH": "pro-inflammatory cytokine cocktail for 18hr"}}, {"GSM118386": {"AGE": "7 years", "STIMULATED WITH": "pro-inflammatory cytokine cocktail for 18hr"}}, {"GSM1183862": {"AGE": "1 year", "STIMULATED WITH": "pro-inflammatory cytokine cocktail for 18hr"}}, {"GSM1183859": {"AGE": "1 year", "STIMULATED WITH": "none (control)"}}, {"GSM1183858": {"AGE": "7 years", "STIMULATED WITH": "none (control)"}}, {"GSM1183859": {"AGE": "1 year", "STIMULATED WITH": "none (control)"}}], "id": 2109, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Human skin-derived precursor cells (hSKP) are a stem cell population that represents key candidates for cell based-therapy. Inflammation,...", "geo_gse_id": "E-GEOD-48757", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 6, "tags": ["cell", "class", "cytokine", "skin", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "pro-inflammatory-stimulation-of-human-skin-derived", "geo_id_plat": "E-GEOD-48757_A-AFFY-44", "name": "Pro-inflammatory stimulation of human skin-derived precursor cells modulates several of their immunology-related pathways.", "created": "Jul.11, 2014", "summary": "Human skin-derived precursor cells (hSKP) are a stem cell population that represents key candidates for cell based-therapy. Inflammation, however, is often present in situations where cellular replacement therapy is required. These inflammatory conditions, and more specifically the presence of the cytokine interferon (IFN)-\u03b3, might result in an increase of MHC class II antigens in hSKP-derived grafts and facilitate their rejection. We used microarray analyses to confirm the activated status of the pro-inflammatory condition, induced by exposure to a cocktail of pro-inflammatory cytokines (IL-1\u03b2, IFN-\u03b3, TNF\u03b1 and IFN-\u03b1). Consequently, we investigated the modulation by inflammation of canonical pathways related to immunology in hSKP. hSKP obtained from 3 different donors (passage 4) were cultivated in the presence or absence of the pro-inflammatory cocktail. Control and pro-inflammatory stimulated samples were collected 18h after treatment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48757", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48757/samples/"}